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| | <StructureSection load='5w95' size='340' side='right'caption='[[5w95]], [[Resolution|resolution]] 1.72Å' scene=''> | | <StructureSection load='5w95' size='340' side='right'caption='[[5w95]], [[Resolution|resolution]] 1.72Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5w95]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W95 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W95 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5w95]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W95 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.723Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ERS007672_01844, ERS023446_02759, ERS024213_00010, ERS024276_01951, ERS027644_01116, ERS027646_00777, ERS027656_01063, ERS027659_00661, ERS027661_01209, ERS027666_00826, ERS031537_00520, ERS124361_01515, SAMEA2683035_00004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w95 OCA], [http://pdbe.org/5w95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w95 RCSB], [http://www.ebi.ac.uk/pdbsum/5w95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w95 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w95 OCA], [https://pdbe.org/5w95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w95 RCSB], [https://www.ebi.ac.uk/pdbsum/5w95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w95 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CULP6_MYCTU CULP6_MYCTU] Shows esterase and phospholipase A activities (PubMed:19169353, PubMed:19225166, PubMed:20656688, PubMed:29247008). May be involved in cell wall biosynthesis and/or maintenance (PubMed:19169353, PubMed:19225166, PubMed:20656688). Can hydrolyze various substrates, including the p-nitrophenol-linked aliphatic esters pNP-laurate (C12), pNP-myristate (C14), pNP-palmitate (C16), pNP-stearate (C18), pNP-butyrate (C4), phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, 4-methylumbelliferyl heptanoate and palmitic acid and arachidonic acid containing phospholipids (PubMed:19169353, PubMed:19225166, PubMed:20656688). Does not exhibit cutinase activity (PubMed:19225166).<ref>PMID:19169353</ref> <ref>PMID:19225166</ref> <ref>PMID:20656688</ref> <ref>PMID:29247008</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Goins, C M]] | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Ronning, D R]] | + | [[Category: Goins CM]] |
| - | [[Category: Schreidah, C M]] | + | [[Category: Ronning DR]] |
| - | [[Category: Complex]] | + | [[Category: Schreidah CM]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Peg]]
| + | |
| Structural highlights
Function
CULP6_MYCTU Shows esterase and phospholipase A activities (PubMed:19169353, PubMed:19225166, PubMed:20656688, PubMed:29247008). May be involved in cell wall biosynthesis and/or maintenance (PubMed:19169353, PubMed:19225166, PubMed:20656688). Can hydrolyze various substrates, including the p-nitrophenol-linked aliphatic esters pNP-laurate (C12), pNP-myristate (C14), pNP-palmitate (C16), pNP-stearate (C18), pNP-butyrate (C4), phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, 4-methylumbelliferyl heptanoate and palmitic acid and arachidonic acid containing phospholipids (PubMed:19169353, PubMed:19225166, PubMed:20656688). Does not exhibit cutinase activity (PubMed:19225166).[1] [2] [3] [4]
Publication Abstract from PubMed
The Mycobacterium tuberculosis (Mtb) rv3802c gene encodes an essential enzyme with thioesterase and phospholipase A activity. Overexpression of Rv3802 orthologs in Mycobacterium smegmatis and Corynebacterium glutamicum increases mycolate content and decreases glycerophospholipids. While a role in modulating the lipid composition of the unique mycomembrane has been proposed, the true biological function of Rv3802 remains uncertain. In this study, we present the first Mtb Rv3802 X-ray crystal structure, solved to 1.7 A resolution. On the basis of the binding of polyethylene glycol (PEG) molecules to Rv3802, we identified its lipid-binding site and the structural basis for phosphatidyl-based substrate binding and phospholipase A activity. We found that movement of the alpha8 helix affords lipid binding and is required for catalytic turnover through covalent tethering. We gained insights into the mechanism of acyl hydrolysis by observing differing arrangements of PEG and water molecules within the active site. This study provides structural insights on biological function and facilitates future structure-based drug design toward Rv3802.
Structural basis for lipid binding and mechanism of the Mycobacterium tuberculosis Rv3802 phospholipase.,Goins CM, Schreidah CM, Dajnowicz S, Ronning DR J Biol Chem. 2017 Dec 15. pii: RA117.000240. doi: 10.1074/jbc.RA117.000240. PMID:29247008[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Parker SK, Barkley RM, Rino JG, Vasil ML. Mycobacterium tuberculosis Rv3802c encodes a phospholipase/thioesterase and is inhibited by the antimycobacterial agent tetrahydrolipstatin. PLoS One. 2009;4(1):e4281. PMID:19169353 doi:10.1371/journal.pone.0004281
- ↑ West NP, Chow FM, Randall EJ, Wu J, Chen J, Ribeiro JM, Britton WJ. Cutinase-like proteins of Mycobacterium tuberculosis: characterization of their variable enzymatic functions and active site identification. FASEB J. 2009 Jun;23(6):1694-704. PMID:19225166 doi:10.1096/fj.08-114421
- ↑ Crellin PK, Vivian JP, Scoble J, Chow FM, West NP, Brammananth R, Proellocks NI, Shahine A, Le Nours J, Wilce MC, Britton WJ, Coppel RL, Rossjohn J, Beddoe T. Tetrahydrolipstatin inhibition, functional analyses, and three-dimensional structure of a lipase essential for mycobacterial viability. J Biol Chem. 2010 Sep 24;285(39):30050-60. PMID:20656688 doi:10.1074/jbc.M110.150094
- ↑ Goins CM, Schreidah CM, Dajnowicz S, Ronning DR. Structural basis for lipid binding and mechanism of the Mycobacterium tuberculosis Rv3802 phospholipase. J Biol Chem. 2017 Dec 15. pii: RA117.000240. doi: 10.1074/jbc.RA117.000240. PMID:29247008 doi:http://dx.doi.org/10.1074/jbc.RA117.000240
- ↑ Goins CM, Schreidah CM, Dajnowicz S, Ronning DR. Structural basis for lipid binding and mechanism of the Mycobacterium tuberculosis Rv3802 phospholipase. J Biol Chem. 2017 Dec 15. pii: RA117.000240. doi: 10.1074/jbc.RA117.000240. PMID:29247008 doi:http://dx.doi.org/10.1074/jbc.RA117.000240
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