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| | <StructureSection load='6heq' size='340' side='right'caption='[[6heq]], [[Resolution|resolution]] 1.23Å' scene=''> | | <StructureSection load='6heq' size='340' side='right'caption='[[6heq]], [[Resolution|resolution]] 1.23Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6heq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabian_camel Arabian camel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HEQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6heq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HEQ FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6heq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6heq OCA], [http://pdbe.org/6heq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6heq RCSB], [http://www.ebi.ac.uk/pdbsum/6heq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6heq ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.23Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6heq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6heq OCA], [https://pdbe.org/6heq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6heq RCSB], [https://www.ebi.ac.uk/pdbsum/6heq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6heq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6heq" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6heq" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Antibody 3D structures|Antibody 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arabian camel]] | + | [[Category: Camelus dromedarius]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Abskharon, R N]] | + | [[Category: Abskharon RN]] |
| - | [[Category: Soror, S H]] | + | [[Category: Soror SH]] |
| - | [[Category: Wohlkonig, A]] | + | [[Category: Wohlkonig A]] |
| - | [[Category: Aggregation]]
| + | |
| - | [[Category: Beta fold]]
| + | |
| - | [[Category: Nanobody]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Prion or PrPSc is the proteinaceous infectious agent causing prion diseases in various mammalian species. Despite decades of research, the structural basis for PrPSc formation and prion infectivity remains elusive. To understand the role of the hydrophobic region in forming infectious prion at the molecular level, we report X-ray crystal structures of mouse (Mo) prion protein (PrP) (residues 89-230) in complex with a nanobody (Nb484). Using the recombinant prion propagation system, we show that the binding of Nb484 to the hydrophobic region of MoPrP efficiently inhibits the propagation of proteinase K resistant PrPSc and prion infectivity. In addition, when added to cultured mouse brain slices in high concentrations, Nb484 exhibits no neurotoxicity, which is drastically different from other neurotoxic anti-PrP antibodies, suggesting that the Nb484 can be a potential therapeutic agent against prion disease. In summary, our data provides the first structure-function evidence supporting a crucial role of the hydrophobic region of PrP in forming an infectious prion.
Structural evidence for the critical role of the prion protein hydrophobic region in forming an infectious prion.,Abskharon R, Wang F, Wohlkonig A, Ruan J, Soror S, Giachin G, Pardon E, Zou W, Legname G, Ma J, Steyaert J PLoS Pathog. 2019 Dec 9;15(12):e1008139. doi: 10.1371/journal.ppat.1008139. PMID:31815959[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Abskharon R, Wang F, Wohlkonig A, Ruan J, Soror S, Giachin G, Pardon E, Zou W, Legname G, Ma J, Steyaert J. Structural evidence for the critical role of the prion protein hydrophobic region in forming an infectious prion. PLoS Pathog. 2019 Dec 9;15(12):e1008139. doi: 10.1371/journal.ppat.1008139. PMID:31815959 doi:http://dx.doi.org/10.1371/journal.ppat.1008139
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