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6qim

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Structure of AtPIP2;4

Structural highlights

6qim is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.7Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PIP24_ARATH Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.

Publication Abstract from PubMed

Aquaporins are membrane-intrinsic proteins initially defined as water (H2O) channels in all organisms and subsequently found to have multiple substrate specificities, such as hydrogen peroxide (H2O2). H2O2 is a signaling molecule that partakes in immune responses where its transport is mediated by aquaporins. To shed further light on the molecular basis of the aquaporin function in H2O2 transport, we have characterized an Arabidopsis thaliana aquaporin, AtPIP2;4, recombinantly produced to high yields in Pichia pastoris. Here, we present a newly established assay that allows detection of H2O2 transport by purified aquaporins reconstituted into liposomes, enabling us to compare aquaporin homologues with respect to substrate specificity. To get additional insight into the structural determinants for aquaporin-mediated H2O2 transport, we solved the 3D-structure of AtPIP2;4 to 3.7A resolution and found structural identity to the water channel from spinach (SoPIP2;1), with the difference that Cd(2+) cation is not required to retain the closed conformation. The transport specificities of the two plant aquaporins were compared to a human homologue, AQP1. Overall, we conclude that AtPIP2;4, SoPIP2;1 and hAQP1 are all transporters of both H2O and H2O2, but have different efficiencies for various specificities. Notably, all three homologues expedite H2O transport equally well while the plant aquaporins are more permeable to H2O2 than hAQP1. Comparison of the structures indicates that the observed variations in H2O and H2O2 transport cannot be explained by differences in the monomeric pore. Possibly, the determinants for transport specificities reside in the flexible domains outside the membrane core of these channels.

Characterization of aquaporin-driven hydrogen peroxide transport.,Wang H, Schoebel S, Schmitz F, Dong H, Hedfalk K Biochim Biophys Acta Biomembr. 2019 Sep 12:183065. doi:, 10.1016/j.bbamem.2019.183065. PMID:31521632^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang H, Schoebel S, Schmitz F, Dong H, Hedfalk K. Characterization of aquaporin-driven hydrogen peroxide transport. Biochim Biophys Acta Biomembr. 2019 Sep 12:183065. doi:, 10.1016/j.bbamem.2019.183065. PMID:31521632 doi:http://dx.doi.org/10.1016/j.bbamem.2019.183065

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6qim"

Categories: Arabidopsis thaliana | Large Structures | Schoebel S | Wang H

@@ Line 3: / Line 3: @@
 <StructureSection load='6qim' size='340' side='right'caption='[[6qim]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6qim]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QIM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6qim]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QIM FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PIP2-4, At5g60660, MUP24.9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qim OCA], [http://pdbe.org/6qim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qim RCSB], [http://www.ebi.ac.uk/pdbsum/6qim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qim ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qim OCA], [https://pdbe.org/6qim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qim RCSB], [https://www.ebi.ac.uk/pdbsum/6qim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qim ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PIP24_ARATH PIP24_ARATH]] Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.
+[https://www.uniprot.org/uniprot/PIP24_ARATH PIP24_ARATH] Aquaporins facilitate the transport of water and small neutral solutes across cell membranes.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
 [[Category: Large Structures]]
-[[Category: Schoebel, S]]
+[[Category: Schoebel S]]
-[[Category: Wang, H]]
+[[Category: Wang H]]
-[[Category: Aquaporin]]
-[[Category: Hydrogen peroxide transport]]
-[[Category: Membrane protein]]

6qim

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Current revision

Structure of AtPIP2;4

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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