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2gnt

From Proteopedia

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Current revision

EDTA treated P. angolensis lectin (PAL) remetallized with calcium (1 hour treatment)

Structural highlights

2gnt is a 2 chain structure with sequence from Pterocarpus angolensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Related:	1ukg, 2gme, 2gmm, 2gmp, 2gn3, 2gnb, 2gnm, 2gnd
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The interplay between metal binding, carbohydrate binding activity, stability and structure of the lectin from Pterocarpus angolensis was investigated. Removal of the metals leads to a more flexible form of the protein with significantly less conformational stability. Crystal structures of this metal-free form show significant structural rearrangements, although some structural features that allow the binding of sugars are retained. We propose that substitution of an asparagine residue at the start of the C-terminal beta-strand of the legume lectin monomer hinders the trans-isomerization of the cis-peptide bond upon demetallization and constitutes an intramolecular switch governing the isomer state of the non-proline bond and ultimately the lectin phenotype.

Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin.,Garcia-Pino A, Buts L, Wyns L, Loris R J Mol Biol. 2006 Aug 4;361(1):153-67. Epub 2006 Jun 21. PMID:16824540^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Garcia-Pino A, Buts L, Wyns L, Loris R. Interplay between metal binding and cis/trans isomerization in legume lectins: structural and thermodynamic study of P. angolensis lectin. J Mol Biol. 2006 Aug 4;361(1):153-67. Epub 2006 Jun 21. PMID:16824540 doi:http://dx.doi.org/10.1016/j.jmb.2006.06.006

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gnt"

@@ Line 3: / Line 3: @@
 <StructureSection load='2gnt' size='340' side='right'caption='[[2gnt]], [[Resolution|resolution]] 2.02&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2gnt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GNT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2gnt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GNT FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ukg|1ukg]], [[2gme|2gme]], [[2gmm|2gmm]], [[2gmp|2gmp]], [[2gn3|2gn3]], [[2gnb|2gnb]], [[2gnm|2gnm]], [[2gnd|2gnd]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ukg|1ukg]], [[2gme|2gme]], [[2gmm|2gmm]], [[2gmp|2gmp]], [[2gn3|2gn3]], [[2gnb|2gnb]], [[2gnm|2gnm]], [[2gnd|2gnd]]</div></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gnt OCA], [http://pdbe.org/2gnt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gnt RCSB], [http://www.ebi.ac.uk/pdbsum/2gnt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gnt ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gnt OCA], [https://pdbe.org/2gnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gnt RCSB], [https://www.ebi.ac.uk/pdbsum/2gnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gnt ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==

2gnt

From Proteopedia

Current revision

EDTA treated P. angolensis lectin (PAL) remetallized with calcium (1 hour treatment)

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

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