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| | <StructureSection load='4xgu' size='340' side='right'caption='[[4xgu]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='4xgu' size='340' side='right'caption='[[4xgu]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xgu]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XGU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xgu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XGU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XGU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pch-2, F10B5.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgu OCA], [https://pdbe.org/4xgu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xgu RCSB], [https://www.ebi.ac.uk/pdbsum/4xgu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xgu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xgu OCA], [http://pdbe.org/4xgu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xgu RCSB], [http://www.ebi.ac.uk/pdbsum/4xgu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xgu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PCH2_CAEEL PCH2_CAEEL]] Plays a key role in chromosome recombination during meiosis. | + | [https://www.uniprot.org/uniprot/PCH2_CAEEL PCH2_CAEEL] Plays a key role in chromosome recombination during meiosis. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Caeel]] | + | [[Category: Caenorhabditis elegans]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Corbett, K D]] | + | [[Category: Corbett KD]] |
| - | [[Category: Ye, Q]] | + | [[Category: Ye Q]] |
| - | [[Category: Aaa+ atpase]]
| + | |
| - | [[Category: Atp-binding protein]]
| + | |
| - | [[Category: Meiotic recombination]]
| + | |
| - | [[Category: Protein remodeler]]
| + | |
| Structural highlights
Function
PCH2_CAEEL Plays a key role in chromosome recombination during meiosis.
Publication Abstract from PubMed
The AAA+ family ATPase TRIP13 is a key regulator of meiotic recombination and the spindle assembly checkpoint, acting on signaling proteins of the conserved HORMA domain family. Here we present the structure of the C. elegans TRIP13 ortholog PCH-2, revealing a new family of AAA+ ATPase protein remodelers. PCH-2 possesses a substrate-recognition domain related to those of the protein remodelers NSF and p97, while its overall hexameric architecture and likely structural mechanism bear close similarities to the bacterial protein unfoldase ClpX. We find that TRIP13, aided by the adapter protein p31(comet), converts the HORMA-family spindle checkpoint protein MAD2 from a signaling-active 'closed' conformer to an inactive 'open' conformer. We propose that TRIP13 and p31(comet) collaborate to inactivate the spindle assembly checkpoint through MAD2 conformational conversion and disassembly of mitotic checkpoint complexes. A parallel HORMA protein disassembly activity likely underlies TRIP13's critical regulatory functions in meiotic chromosome structure and recombination.
TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching.,Ye Q, Rosenberg SC, Moeller A, Speir JA, Su TY, Corbett KD Elife. 2015 Apr 28;4. doi: 10.7554/eLife.07367. PMID:25918846[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ye Q, Rosenberg SC, Moeller A, Speir JA, Su TY, Corbett KD. TRIP13 is a protein-remodeling AAA+ ATPase that catalyzes MAD2 conformation switching. Elife. 2015 Apr 28;4. doi: 10.7554/eLife.07367. PMID:25918846 doi:http://dx.doi.org/10.7554/eLife.07367
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