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5c74

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Revision as of 06:22, 7 June 2023

Structure of a novel protein arginine methyltransferase

Structural highlights

5c74 is a 2 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SFM1_YEAST S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that monomethylates ribosomal protein S3 (RPS3) at 'Arg-146'.^[1]

Publication Abstract from PubMed

SPOUT proteins constitute one class of methyltransferases, which so far are found to exert activity mainly towards RNAs. Previously, yeast Sfm1 was predicted to contain a SPOUT domain but can methylate ribosomal protein S3. Here we report the crystal structure of Sfm1, which comprises of a typical SPOUT domain and a small C-terminal domain. The active site is similar to that of protein arginine methyltransferases but different from that of RNA methyltransferases. In addition, Sfm1 exhibits a negatively charged surface surrounding the active site unsuitable for RNA binding. Our biochemical data show that Sfm1 exists as a monomer and has high activity towards ribosomal protein S3 but no activity towards RNA. It can specifically catalyze the methylation of Arg146 of S3 and the C-terminal domain is critical for substrate binding and activity. These results together provide the structural basis for Sfm1 functioning as a PRMT for ribosomal protein S3.

Structural basis for Sfm1 functioning as a protein arginine methyltransferase.,Lv F, Zhang T, Zhou Z, Gao S, Wong CC, Zhou JQ, Ding J Cell Discov. 2015 Dec 29;1:15037. doi: 10.1038/celldisc.2015.37. eCollection, 2015. PMID:27462434^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Young BD, Weiss DI, Zurita-Lopez CI, Webb KJ, Clarke SG, McBride AE. Identification of methylated proteins in the yeast small ribosomal subunit: a role for SPOUT methyltransferases in protein arginine methylation. Biochemistry. 2012 Jun 26;51(25):5091-104. doi: 10.1021/bi300186g. Epub 2012 Jun , 15. PMID:22650761 doi:http://dx.doi.org/10.1021/bi300186g
↑ Lv F, Zhang T, Zhou Z, Gao S, Wong CC, Zhou JQ, Ding J. Structural basis for Sfm1 functioning as a protein arginine methyltransferase. Cell Discov. 2015 Dec 29;1:15037. doi: 10.1038/celldisc.2015.37. eCollection, 2015. PMID:27462434 doi:http://dx.doi.org/10.1038/celldisc.2015.37

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5c74"

Categories: Large Structures | Saccharomyces cerevisiae S288C | Ding J | Lv F

@@ Line 3: / Line 3: @@
 <StructureSection load='5c74' size='340' side='right'caption='[[5c74]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5c74]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C74 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5c74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C74 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5c77|5c77]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c74 OCA], [https://pdbe.org/5c74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c74 RCSB], [https://www.ebi.ac.uk/pdbsum/5c74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c74 ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SFM1, YOR021C, OR26.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c74 OCA], [http://pdbe.org/5c74 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c74 RCSB], [http://www.ebi.ac.uk/pdbsum/5c74 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c74 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SFM1_YEAST SFM1_YEAST]] S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that monomethylates ribosomal protein S3 (RPS3) at 'Arg-146'.<ref>PMID:22650761</ref>
+[https://www.uniprot.org/uniprot/SFM1_YEAST SFM1_YEAST] S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that monomethylates ribosomal protein S3 (RPS3) at 'Arg-146'.<ref>PMID:22650761</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Baker's yeast]]
 [[Category: Large Structures]]
-[[Category: Ding, J]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Lv, F]]
+[[Category: Ding J]]
-[[Category: Binging seite]]
+[[Category: Lv F]]
-[[Category: Protein arginine methyltransferase]]
-[[Category: S-adenosylhomocysteine]]
-[[Category: Transferase]]

5c74

From Proteopedia

Revision as of 06:22, 7 June 2023

Structure of a novel protein arginine methyltransferase

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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