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| | <StructureSection load='5ehk' size='340' side='right'caption='[[5ehk]], [[Resolution|resolution]] 2.71Å' scene=''> | | <StructureSection load='5ehk' size='340' side='right'caption='[[5ehk]], [[Resolution|resolution]] 2.71Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ehk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-20 Atcc baa-20]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EHK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ehk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbispora_corallina Microbispora corallina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EHK FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mibB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83302 ATCC BAA-20])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.708Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ehk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ehk OCA], [http://pdbe.org/5ehk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ehk RCSB], [http://www.ebi.ac.uk/pdbsum/5ehk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ehk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ehk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ehk OCA], [https://pdbe.org/5ehk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ehk RCSB], [https://www.ebi.ac.uk/pdbsum/5ehk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ehk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/E2IHB7_9ACTN E2IHB7_9ACTN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc baa-20]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hao, Y]]
| + | [[Category: Microbispora corallina]] |
| - | [[Category: Nair, S K]]
| + | [[Category: Hao Y]] |
| - | [[Category: Hydrolase]]
| + | [[Category: Nair SK]] |
| - | [[Category: Lantibiotic dehydratase]]
| + | |
| - | [[Category: Microbispora sp. 107891]] | + | |
| - | [[Category: Nai-107]] | + | |
| - | [[Category: Trna dependent]] | + | |
| Structural highlights
Function
E2IHB7_9ACTN
Publication Abstract from PubMed
Class I lantibiotic dehydratases dehydrate selected Ser/Thr residues of a precursor peptide. Recent studies demonstrated the requirement of glutamyl-tRNAGlu for Ser/Thr activation by one of these enzymes (NisB) from the Firmicute Lactococcus lactis. However, the generality of glutamyl-tRNAGlu usage and the tRNA specificity of lantibiotic dehydratases have not been established. Here we report the 2.7-A resolution crystal structure, along with the glutamyl-tRNAGlu utilization of MibB, a lantibiotic dehydratase from the Actinobacterium Microbispora sp. 107891 involved in the biosynthesis of the clinical candidate NAI-107. Biochemical assays revealed nucleotides A73 and U72 within the tRNAGlu acceptor stem to be important for MibB glutamyl-tRNAGlu usage. Using this knowledge, an expression system for the production of NAI-107 analogs in Escherichia coli was developed, overcoming the inability of MibB to utilize E. coli tRNAGlu. Our work provides evidence for a common tRNAGlu-dependent dehydration mechanism, paving the way for the characterization of lantibiotics from various phyla.
Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis.,Ortega MA, Hao Y, Walker MC, Donadio S, Sosio M, Nair SK, van der Donk WA Cell Chem Biol. 2016 Feb 8. pii: S2451-9456(16)30001-0. doi:, 10.1016/j.chembiol.2015.11.017. PMID:26877024[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ortega MA, Hao Y, Walker MC, Donadio S, Sosio M, Nair SK, van der Donk WA. Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis. Cell Chem Biol. 2016 Feb 8. pii: S2451-9456(16)30001-0. doi:, 10.1016/j.chembiol.2015.11.017. PMID:26877024 doi:http://dx.doi.org/10.1016/j.chembiol.2015.11.017
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