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5ikl

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Revision as of 13:58, 30 August 2023

Crystal structure of P. aeruginosa geranyl-CoA carboxylase (GCC), beta subunit

Structural highlights

5ikl is a 3 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.4Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9HZV9_PSEAE

Publication Abstract from PubMed

Geranyl-CoA carboxylase (GCC) is essential for the growth of Pseudomonas organisms with geranic acid as the sole carbon source. GCC has the same domain organization and shares strong sequence conservation with the related biotin-dependent carboxylases 3-methylcrotonyl-CoA carboxylase (MCC) and propionyl-CoA carboxylase (PCC). Here we report the crystal structure of the 750-kDa alpha6beta6 holoenzyme of GCC, which is similar to MCC but strikingly different from PCC. The structures provide evidence in support of two distinct lineages of biotin-dependent acyl-CoA carboxylases, one carboxylating the alpha carbon of a saturated organic acid and the other carboxylating the gamma carbon of an alpha-beta unsaturated acid. Structural differences in the active site region of GCC and MCC explain their distinct substrate preferences. Especially, a glycine residue in GCC is replaced by phenylalanine in MCC, which blocks access by the larger geranyl-CoA substrate. Mutation of this residue in the two enzymes can change their substrate preferences.

Structure and substrate selectivity of the 750-kDa alpha6beta6 holoenzyme of geranyl-CoA carboxylase.,Jurado AR, Huang CS, Zhang X, Zhou ZH, Tong L Nat Commun. 2015 Nov 23;6:8986. doi: 10.1038/ncomms9986. PMID:26593090^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jurado AR, Huang CS, Zhang X, Zhou ZH, Tong L. Structure and substrate selectivity of the 750-kDa alpha6beta6 holoenzyme of geranyl-CoA carboxylase. Nat Commun. 2015 Nov 23;6:8986. doi: 10.1038/ncomms9986. PMID:26593090 doi:http://dx.doi.org/10.1038/ncomms9986

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ikl"

Categories: Large Structures | Pseudomonas aeruginosa PAO1 | Huang CS | Jurado AR | Tong L

@@ Line 3: / Line 3: @@
 <StructureSection load='5ikl' size='340' side='right'caption='[[5ikl]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ikl]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IKL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ikl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IKL FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">atuC, PA2888 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ikl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikl OCA], [http://pdbe.org/5ikl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ikl RCSB], [http://www.ebi.ac.uk/pdbsum/5ikl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ikl ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ikl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ikl OCA], [https://pdbe.org/5ikl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ikl RCSB], [https://www.ebi.ac.uk/pdbsum/5ikl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ikl ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9HZV9_PSEAE Q9HZV9_PSEAE]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Pseae]]
+[[Category: Pseudomonas aeruginosa PAO1]]
-[[Category: Huang, C S]]
+[[Category: Huang CS]]
-[[Category: Jurado, A R]]
+[[Category: Jurado AR]]
-[[Category: Tong, L]]
+[[Category: Tong L]]
-[[Category: Carboxylase]]
-[[Category: Ligase]]
-[[Category: Organic acid metabolism]]

5ikl

From Proteopedia

Revision as of 13:58, 30 August 2023

Crystal structure of P. aeruginosa geranyl-CoA carboxylase (GCC), beta subunit

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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