We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

5w1a

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:13, 13 March 2024) (edit) (undo)
 
Line 3: Line 3:
<StructureSection load='5w1a' size='340' side='right'caption='[[5w1a]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
<StructureSection load='5w1a' size='340' side='right'caption='[[5w1a]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5w1a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4qbd 4qbd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W1A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W1A FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5w1a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4qbd 4qbd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W1A FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.227&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mhc, CG17927 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME]), Mlc1, MLC-ALK, CG5596 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w1a OCA], [http://pdbe.org/5w1a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w1a RCSB], [http://www.ebi.ac.uk/pdbsum/5w1a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w1a ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w1a OCA], [https://pdbe.org/5w1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w1a RCSB], [https://www.ebi.ac.uk/pdbsum/5w1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w1a ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/MYSA_DROME MYSA_DROME]] Muscle contraction.
+
[https://www.uniprot.org/uniprot/MYSA_DROME MYSA_DROME] Muscle contraction.
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Drosophila melanogaster is a powerful system for characterizing alternative myosin isoforms and modeling muscle diseases, but high-resolution structures of fruit fly contractile proteins have not been determined. Here we report the first x-ray crystal structure of an insect myosin: the D. melanogaster skeletal muscle myosin II embryonic isoform (EMB). Using our system for recombinant expression of myosin heavy chain (MHC) proteins in whole transgenic flies, we prepared and crystallized stable proteolytic S1-like fragments containing the entire EMB motor domain bound to an essential light chain. We solved the x-ray crystal structure by molecular replacement and refined the resulting model against diffraction data to 2.2 A resolution. The protein is captured in two slightly different renditions of the rigor-like conformation with a citrate of crystallization at the nucleotide binding site and exhibits structural features common to myosins of diverse classes from all kingdoms of life. All atom molecular dynamics simulations on EMB in its nucleotide-free state and a derivative homology model containing 61 amino acid substitutions unique to the indirect flight muscle isoform (IFI) suggest that differences in the identity of residues within the relay and the converter that are encoded for by MHC alternative exons 9 and 11, respectively, directly contribute to increased mobility of these regions in IFI relative to EMB. This suggests the possibility that alternative folding or conformational stability within these regions contribute to the observed functional differences in Drosophila EMB and IFI myosins.
+
-
 
+
-
X-ray crystallographic and molecular dynamic analyses of Drosophila melanogaster embryonic muscle myosin define domains responsible for isoform-specific properties.,Caldwell JT, Mermelstein DJ, Walker RC, Bernstein SI, Huxford T J Mol Biol. 2019 Nov 28. pii: S0022-2836(19)30678-3. doi:, 10.1016/j.jmb.2019.11.013. PMID:31786266<ref>PMID:31786266</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5w1a" style="background-color:#fffaf0;"></div>
+
==See Also==
==See Also==
*[[Myosin 3D Structures|Myosin 3D Structures]]
*[[Myosin 3D Structures|Myosin 3D Structures]]
-
== References ==
 
-
<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Drome]]
+
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Large Structures]]
-
[[Category: Bernstein, S I]]
+
[[Category: Bernstein SI]]
-
[[Category: Caldwell, J T]]
+
[[Category: Caldwell JT]]
-
[[Category: Huxford, T]]
+
[[Category: Huxford T]]
-
[[Category: Embryonic isoform]]
+
-
[[Category: Indirect flight muscle]]
+
-
[[Category: Insect]]
+
-
[[Category: Motor protein]]
+
-
[[Category: Skeletal muscle myosin ii]]
+
-
[[Category: Subfragment-1]]
+

Current revision

The first X-ray crystal structure of an insect muscle myosin. Drosophila melanogaster, skeletal muscle myosin II, an embryonic isoform, subfragment-1

PDB ID 5w1a

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5w1a"
Personal tools