From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:1a73.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1a73.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_1a73| PDB=1a73 | SCENE= }} | | {{STRUCTURE_1a73| PDB=1a73 | SCENE= }} |
| | | | |
| - | '''INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA'''
| + | ===INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | Homing endonucleases are a diverse collection of proteins that are encoded by genes with mobile, self-splicing introns. They have also been identified in self-splicing inteins (protein introns). These enzymes promote the movement of the DNA sequences that encode them from one chromosome location to another; they do this by making a site-specific double-strand break at a target site in an allele that lacks the corresponding mobile intron. The target sites recognized by these small endonucleases are generally long (14-44 base pairs). Four families of homing endonucleases have been identified, including the LAGLIDADG, the His-Cys box, the GIY-YIG and the H-N-H endonucleases. The first identified His-Cys box homing endonuclease was I-PpoI from the slime mould Physarum polycephalum. Its gene resides in one of only a few nuclear introns known to exhibit genetic mobility. Here we report the structure of the I-PpoI homing endonuclease bound to homing-site DNA determined to 1.8 A resolution. I-PpoI displays an elongated fold of dimensions 25 x 35 x 80 A, with mixed alpha/beta topology. Each I-PpoI monomer contains three antiparallel beta-sheets flanked by two long alpha-helices and a long carboxy-terminal tail, and is stabilized by two bound zinc ions 15 A apart. The enzyme possesses a new zinc-bound fold and endonuclease active site. The structure has been determined in both uncleaved substrate and cleaved product complexes.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9665136}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9665136 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_9665136}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 29: |
Line 33: |
| | [[Category: Protein folding]] | | [[Category: Protein folding]] |
| | [[Category: Zinc]] | | [[Category: Zinc]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 09:54:47 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:17:06 2008'' |
Revision as of 13:17, 30 June 2008
Template:STRUCTURE 1a73
INTRON-ENCODED ENDONUCLEASE I-PPOI COMPLEXED WITH DNA
Template:ABSTRACT PUBMED 9665136
About this Structure
1A73 is a Single protein structure of sequence from Physarum polycephalum. Full crystallographic information is available from OCA.
Reference
DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI., Flick KE, Jurica MS, Monnat RJ Jr, Stoddard BL, Nature. 1998 Jul 2;394(6688):96-101. PMID:9665136
Page seeded by OCA on Mon Jun 30 16:17:06 2008
