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| | <StructureSection load='5kk8' size='340' side='right'caption='[[5kk8]], [[Resolution|resolution]] 2.11Å' scene=''> | | <StructureSection load='5kk8' size='340' side='right'caption='[[5kk8]], [[Resolution|resolution]] 2.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5kk8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Blood_fluke Blood fluke]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KK8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5kk8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KK8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5iom|5iom]], [[5iol|5iol]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Smp_092750 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6183 Blood fluke])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk8 OCA], [https://pdbe.org/5kk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kk8 RCSB], [https://www.ebi.ac.uk/pdbsum/5kk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk8 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk8 OCA], [http://pdbe.org/5kk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kk8 RCSB], [http://www.ebi.ac.uk/pdbsum/5kk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Blood fluke]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Nucleoside-diphosphate kinase]] | + | [[Category: Schistosoma mansoni]] |
| - | [[Category: Aller, P]] | + | [[Category: Aller P]] |
| - | [[Category: Bird, L E]] | + | [[Category: Bird LE]] |
| - | [[Category: Brandao-Neto, J]] | + | [[Category: Brandao-Neto J]] |
| - | [[Category: DeMarco, R]] | + | [[Category: DeMarco R]] |
| - | [[Category: Nettleship, J E]] | + | [[Category: Nettleship JE]] |
| - | [[Category: Owens, R J]] | + | [[Category: Owens RJ]] |
| - | [[Category: Pereira, H M]] | + | [[Category: Pereira HM]] |
| - | [[Category: Romanello, L]] | + | [[Category: Romanello L]] |
| - | [[Category: Torini, J R.S]] | + | [[Category: Torini JRS]] |
| - | [[Category: Enzyme]]
| + | |
| - | [[Category: Nucleoside diphosphate kinase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Nucleoside diphosphate kinases (NDPKs) are crucial to keep the high triphosphate nucleotide levels in the biological process. The enzymatic mechanism has been extensively described; however, the structural characteristics and kinetic parameters have never been fully determined. In Schistosoma mansoni, NDPK (SmNDPK) is directly involved in the pyrimidine and purine salvage pathways, being essential for nucleotide metabolism. The SmNDPK enzymatic activity is the highest of the known purine metabolisms when compared to the mammalian NDPKs, suggesting the importance of this enzyme in the worm metabolism. Here, we report the recombinant expression of SmNDPK that resulted in 1.7 and 1.9 A apo-form structure in different space-groups, as well as the 2.1 A SmNDPK.ADP complex. The binding and kinetic assays reveal the ATP-dependence for enzyme activation. Moreover, in situ hybridization showed that SmNDPK transcripts are found in reproductive organs and in the esophagus gland of adult worms, which can be intrinsically related with the oviposition and digestive processes. These results will help us fully understand the crucial participation of this enzyme in Schistosoma mansoni and its importance for the pathology of the disease.
Characterization of a Schistosoma mansoni NDPK expressed in sexual and digestive organs.,Torini JR, de Freitas Fernandes A, Balasco Serrao VH, Romanello L, Bird LE, Nettleship JE, Owens RJ, Brandao-Neto J, Zeraik AE, DeMarco R, D'Muniz Pereira H Mol Biochem Parasitol. 2019 May 16;231:111187. doi:, 10.1016/j.molbiopara.2019.111187. PMID:31103556[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Torini JR, de Freitas Fernandes A, Balasco Serrao VH, Romanello L, Bird LE, Nettleship JE, Owens RJ, Brandao-Neto J, Zeraik AE, DeMarco R, D'Muniz Pereira H. Characterization of a Schistosoma mansoni NDPK expressed in sexual and digestive organs. Mol Biochem Parasitol. 2019 May 16;231:111187. doi:, 10.1016/j.molbiopara.2019.111187. PMID:31103556 doi:http://dx.doi.org/10.1016/j.molbiopara.2019.111187
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