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| | <StructureSection load='6nmj' size='340' side='right'caption='[[6nmj]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='6nmj' size='340' side='right'caption='[[6nmj]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nmj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NMJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NMJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nmj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NMJ FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6nmg|6nmg]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ric8a, rCG_48458 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nmj OCA], [https://pdbe.org/6nmj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nmj RCSB], [https://www.ebi.ac.uk/pdbsum/6nmj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nmj ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nmj OCA], [http://pdbe.org/6nmj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nmj RCSB], [http://www.ebi.ac.uk/pdbsum/6nmj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nmj ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B1H241_RAT B1H241_RAT] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Buffalo rat]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mou, T C]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Sprang, S R]] | + | [[Category: Mou TC]] |
| - | [[Category: Zeng, B]] | + | [[Category: Sprang SR]] |
| - | [[Category: Signaling protein]] | + | [[Category: Zeng B]] |
| Structural highlights
Function
B1H241_RAT
Publication Abstract from PubMed
Ric-8A is a 530-amino acid cytoplasmic molecular chaperone and guanine nucleotide exchange factor (GEF) for i, q, and 12/13 classes of heterortrimeric G protein alpha subunits (Galpha). We report the 2.2-A crystal structure of the Ric-8A Galpha-binding domain with GEF activity, residues 1-452, and is phosphorylated at Ser435 and Thr440. Residues 1-429 adopt a superhelical fold comprised of Armadillo (ARM) and HEAT repeats, and the C terminus is disordered. One of the phosphorylated residues potentially binds to a basic cluster in an ARM motif. Amino acid sequence conservation and published hydrogen-deuterium exchange data indicate repeats 3 through 6 to be a putative Galpha-binding surface. Normal mode modeling of small-angle X-ray scattering data indicates that phosphorylation induces relative rotation between repeats 1-4, 5-6, and 7-9. 2D (1)H-(15)N-TROSY spectra of [(2)H,(15)N]-labeled Galphai1 in the presence of R452 reveals chemical shift perturbations of the C terminus and Galphai1 residues involved in nucleotide binding.
Structure, Function, and Dynamics of the Galpha Binding Domain of Ric-8A.,Zeng B, Mou TC, Doukov TI, Steiner A, Yu W, Papasergi-Scott M, Tall GG, Hagn F, Sprang SR Structure. 2019 Jul 2;27(7):1137-1147.e5. doi: 10.1016/j.str.2019.04.013. Epub, 2019 May 30. PMID:31155309[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zeng B, Mou TC, Doukov TI, Steiner A, Yu W, Papasergi-Scott M, Tall GG, Hagn F, Sprang SR. Structure, Function, and Dynamics of the Galpha Binding Domain of Ric-8A. Structure. 2019 Jul 2;27(7):1137-1147.e5. doi: 10.1016/j.str.2019.04.013. Epub, 2019 May 30. PMID:31155309 doi:http://dx.doi.org/10.1016/j.str.2019.04.013
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