Structural insights into conformational switching in latency-associated peptide between TGFβ-1 bound and unbound states
Timothy R. Stachowski, Mary E. Snell, and Edward H. Snell [1]
Molecular Tour
In response to environmental changes, proteins are released from cells that act as messengers, which allows cells to communicate with one another and coordinate a physiologic response. Transforming growth factor β-1 (TGFβ-1) is a protein used to communicate information about when and how cells should proliferate and is particularly important in organizing embryonic tissue development. The fundamental way our cells regulate TGFβ-1 activity is with a second protein, latency-associated peptide (LAP). LAP forms a complex with TGFβ-1, tethering it to the outside surface of the cell. Several pathologies including cancer progression and immune suppression are driven by an overabundance of TGFβ-1.
Because LAP binding reduces TGFβ-1 activity, recombinant LAP is a promising novel therapeutic approach, however a detailed understanding of how LAP binds TGFβ-1 is missing, which could reveal ways to improve the LAP-TGFβ-1 interaction or develop other anti-TGFβ-1 therapies. In a paper in IUCrJ, Stachowski et al. show that LAP adopts structurally distinct conformations between unbound and TGFβ-1 bound states. This was revealed by comparing the X-ray crystal structure of LAP in the unbound state (6p7j) solved by Stachowski et al. with the crystal structure of LAP bound to TGFβ-1 (3rjr) previously reported by Shi et al. 2011 [2]. This analysis combined with solution modelling showed that LAP transitions from an extended to compact conformation when binding TGFβ-1, simulating a type of hugging motion where LAP embraces TGFβ-1. Surprisingly, this conformational change includes rearranging two domains that are distant from one another and might be coordinated through the formation of an α-helix that is distant from the LAP-TGFβ-1 interaction site. Together, these results provide new spatial details about the TGFβ-1 binding mechanism, specifically how LAP reorients itself to develop the LAP-TGFβ-1 interface. This insight is important as it provides new avenues to engineer and improve LAP as a therapeutic that are focused beyond just the interaction site.
References
- ↑ Stachowski TR, Snell ME, Snell EH. Structural insights into conformational switching in latency-associated peptide between transforming growth factor beta-1 bound and unbound states. IUCrJ. 2020 Feb 6;7(Pt 2):238-252. doi: 10.1107/S205225251901707X. eCollection, 2020 Mar 1. PMID:32148852 doi:http://dx.doi.org/10.1107/S205225251901707X
- ↑ Shi M, Zhu J, Wang R, Chen X, Mi L, Walz T, Springer TA. Latent TGF-beta structure and activation. Nature. 2011 Jun 15;474(7351):343-9. doi: 10.1038/nature10152. PMID:21677751 doi:http://dx.doi.org/10.1038/nature10152
