6tte
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Beta-galactosidase in complex with PETG== | |
| + | <SX load='6tte' size='340' side='right' viewer='molstar' caption='[[6tte]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6tte]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TTE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TTE FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PTQ:2-PHENYLETHYL+1-THIO-BETA-D-GALACTOPYRANOSIDE'>PTQ</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lacZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tte OCA], [http://pdbe.org/6tte PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tte RCSB], [http://www.ebi.ac.uk/pdbsum/6tte PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tte ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Recent advances in cryo-electron microscopy (EM) structure determination have pushed the resolutions obtainable by the method into the range widely considered to be of utility for drug discovery. Here, we review the use of cryo-EM in fragment-based drug discovery (FBDD) based on in-house method development. We demonstrate not only that cryo-EM can reveal details of the molecular interactions between fragments and a protein, but also that the current reproducibility, quality, and throughput are compatible with FBDD. We exemplify this using the test system beta-galactosidase (Bgal) and the oncology target pyruvate kinase 2 (PKM2). | ||
| - | + | Fragment-based drug discovery using cryo-EM.,Saur M, Hartshorn MJ, Dong J, Reeks J, Bunkoczi G, Jhoti H, Williams PA Drug Discov Today. 2019 Dec 23. pii: S1359-6446(19)30465-9. doi:, 10.1016/j.drudis.2019.12.006. PMID:31877353<ref>PMID:31877353</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6tte" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </SX> | ||
| + | [[Category: Bacillus coli migula 1895]] | ||
| + | [[Category: Beta-galactosidase]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bunkoczi, G]] | ||
| + | [[Category: Dong, J]] | ||
| + | [[Category: Hartshorn, M J]] | ||
| + | [[Category: Jhoti, H]] | ||
| + | [[Category: Reeks, J]] | ||
| + | [[Category: Saur, M]] | ||
| + | [[Category: Williams, P A]] | ||
| + | [[Category: Bgal]] | ||
| + | [[Category: Petg]] | ||
| + | [[Category: Sugar binding protein]] | ||
Revision as of 01:02, 7 March 2020
Beta-galactosidase in complex with PETG
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