1a99

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{{STRUCTURE_1a99| PDB=1a99 | SCENE= }}
{{STRUCTURE_1a99| PDB=1a99 | SCENE= }}
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'''PUTRESCINE RECEPTOR (POTF) FROM E. COLI'''
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===PUTRESCINE RECEPTOR (POTF) FROM E. COLI===
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==Overview==
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PotF protein is a periplasmic substrate-binding protein of the putrescine transport system in Escherichia coli. We have determined the crystal structure of PotF protein in complex with the substrate at 2.3-A resolution. The PotF molecule has dimensions of 54 x 42 x 30 A and consists of two similar globular domains. The PotF structure is reminiscent of other periplasmic receptors with a highest structural homology to another polyamine-binding protein, PotD. Putrescine is tightly bound in the deep cleft between the two domains of PotF through 12 hydrogen bonds and 36 van der Waals interactions. The comparison of the PotF structure with that of PotD provides the insight into the differences in the specificity between the two proteins. The PotF structure, in combination with the mutational analysis, revealed the residues crucial for putrescine binding (Trp-37, Ser-85, Glu-185, Trp-244, Asp-247, and Asp-278) and the importance of water molecules for putrescine recognition.
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The line below this paragraph, {{ABSTRACT_PUBMED_9651355}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9651355 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9651355}}
==About this Structure==
==About this Structure==
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[[Category: Binding protein]]
[[Category: Binding protein]]
[[Category: Transport]]
[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:00:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:25:35 2008''

Revision as of 13:25, 30 June 2008

Image:1a99.png

Template:STRUCTURE 1a99

PUTRESCINE RECEPTOR (POTF) FROM E. COLI

Template:ABSTRACT PUBMED 9651355

About this Structure

1A99 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure and mutational analysis of the Escherichia coli putrescine receptor. Structural basis for substrate specificity., Vassylyev DG, Tomitori H, Kashiwagi K, Morikawa K, Igarashi K, J Biol Chem. 1998 Jul 10;273(28):17604-9. PMID:9651355

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