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| | <StructureSection load='5bu6' size='340' side='right'caption='[[5bu6]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='5bu6' size='340' side='right'caption='[[5bu6]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5bu6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alcaligenes_bronchisepticus Alcaligenes bronchisepticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BU6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BU6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5bu6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_bronchiseptica_RB50 Bordetella bronchiseptica RB50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BU6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BU6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hmsF, BN113_1689, BB1768 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=257310 Alcaligenes bronchisepticus])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bu6 OCA], [https://pdbe.org/5bu6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bu6 RCSB], [https://www.ebi.ac.uk/pdbsum/5bu6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bu6 ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetylglucosamine_deacetylase N-acetylglucosamine deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.33 3.5.1.33] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bu6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bu6 OCA], [http://pdbe.org/5bu6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bu6 RCSB], [http://www.ebi.ac.uk/pdbsum/5bu6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bu6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Alcaligenes bronchisepticus]] | + | [[Category: Bordetella bronchiseptica RB50]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: N-acetylglucosamine deacetylase]]
| + | [[Category: Bamford NC]] |
| - | [[Category: Bamford, N C]] | + | [[Category: Howell PL]] |
| - | [[Category: Howell, P L]] | + | [[Category: Little DJ]] |
| - | [[Category: Little, D J]] | + | |
| - | [[Category: Deacetylase]]
| + | |
| - | [[Category: Family 4 carbohydrate esterase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Bordetella pertussis and Bordetella bronchiseptica are the causative agents of whooping cough in humans and a variety of respiratory diseases in animals, respectively. Bordetella species produce an exopolysaccharide, known as the Bordetella polysaccharide (Bps), which is encoded by the bpsABCD operon. Bps is required for Bordetella biofilm formation, colonization of the respiratory tract, and confers protection from complement-mediated killing. In this report, we have investigated the role of BpsB in the biosynthesis of Bps and biofilm formation by B. bronchiseptica. BpsB is a two-domain protein that localizes to the periplasm and outer membrane. BpsB displays metal- and length-dependent deacetylation on poly-beta-(1,6)-N-acetyl-D-glucosamine (PNAG) oligomers, supporting previous immunogenic data that suggests Bps is a PNAG polymer. BpsB can use a variety of divalent metal cations for deacetylase activity and showed highest activity in the presence of Ni2+ and Co2+. The structure of BpsB's deacetylase domain is similar to the PNAG deacetylases PgaB and IcaB, and contains the same circularly permuted family four carbohydrate esterase motifs. Unlike PgaB from E. coli, BpsB is not required for polymer export and has unique structural differences that allow the N-terminal deacetylase domain to be active when purified in isolation from the C-terminal domain. Our enzymatic characterizations highlight the importance of conserved active site residues in PNAG deacetylation and demonstrate that the C-terminal domain is required for maximal deacetylation of longer PNAG oligomers. Furthermore, we show that BpsB is critical for the formation and complex architecture of B. bronchiseptica biofilms.
BpsB is a Poly-beta-1,6-N-acetyl-D-glucosamine Deacetylase Required for Biofilm Formation in Bordetella bronchiseptica.,Little DJ, Milek S, Bamford NC, Ganguly T, DiFrancesco BR, Nitz M, Deora R, Howell PL J Biol Chem. 2015 Jul 22. pii: jbc.M115.672469. PMID:26203190[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Little DJ, Milek S, Bamford NC, Ganguly T, DiFrancesco BR, Nitz M, Deora R, Howell PL. BpsB is a Poly-beta-1,6-N-acetyl-D-glucosamine Deacetylase Required for Biofilm Formation in Bordetella bronchiseptica. J Biol Chem. 2015 Jul 22. pii: jbc.M115.672469. PMID:26203190 doi:http://dx.doi.org/10.1074/jbc.M115.672469
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