|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5klh' size='340' side='right'caption='[[5klh]], [[Resolution|resolution]] 1.65Å' scene=''> | | <StructureSection load='5klh' size='340' side='right'caption='[[5klh]], [[Resolution|resolution]] 1.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5klh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_(trypanozoon)_brucei Trypanosoma (trypanozoon) brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KLH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5klh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KLH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.646Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5klh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5klh OCA], [http://pdbe.org/5klh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5klh RCSB], [http://www.ebi.ac.uk/pdbsum/5klh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5klh ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5klh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5klh OCA], [https://pdbe.org/5klh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5klh RCSB], [https://www.ebi.ac.uk/pdbsum/5klh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5klh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q26806_9TRYP Q26806_9TRYP] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 24: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boulanger, M J]] | + | [[Category: Trypanosoma brucei]] |
| - | [[Category: Parker, M L]] | + | [[Category: Boulanger MJ]] |
| - | [[Category: Ramaswamy, R]] | + | [[Category: Parker ML]] |
| - | [[Category: Bi-lobed architecture]] | + | [[Category: Ramaswamy R]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Putative sensor]]
| + | |
| - | [[Category: Surface protein]]
| + | |
| Structural highlights
Function
Q26806_9TRYP
Publication Abstract from PubMed
African trypanosomiasis, caused by parasites of the genus Trypanosoma, is a complex of devastating vector-borne diseases of humans and livestock in sub-Saharan Africa. Central to the pathogenesis of African trypanosomes is their transmission by the arthropod vector, Glossina spp. (tsetse fly). Intriguingly, the efficiency of parasite transmission through the vector is reduced following depletion of Trypanosoma brucei Procyclic-Specific Surface Antigen-2 (TbPSSA-2). To investigate the underlying molecular mechanism of TbPSSA-2, we determined the crystal structures of its ectodomain and that of its homolog T. congolense Insect Stage Antigen (TcISA) to resolutions of 1.65 A and 2.45 A, respectively using single wavelength anomalous dispersion. Both proteins adopt a novel bilobed architecture with the individual lobes displaying rotational flexibility around the central tether that suggest a potential mechanism for coordinating a binding partner. In support of this hypothesis, electron density consistent with a bound peptide was observed in the inter-lob cleft of a TcISA monomer. These first reported structures of insect stage transmembrane proteins expressed by African trypanosomes provide potentially valuable insight into the interface between parasite and tsetse vector.
Structural characterization reveals a novel bilobed architecture for the ectodomains of insect stage expressed Trypanosoma brucei PSSA-2 and Trypanosoma congolense ISA.,Ramaswamy R, Goomeshi Nobary S, Eyford BA, Pearson TW, Boulanger MJ Protein Sci. 2016 Dec;25(12):2297-2302. doi: 10.1002/pro.3053. Epub 2016 Oct 13. PMID:27671214[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ramaswamy R, Goomeshi Nobary S, Eyford BA, Pearson TW, Boulanger MJ. Structural characterization reveals a novel bilobed architecture for the ectodomains of insect stage expressed Trypanosoma brucei PSSA-2 and Trypanosoma congolense ISA. Protein Sci. 2016 Dec;25(12):2297-2302. doi: 10.1002/pro.3053. Epub 2016 Oct 13. PMID:27671214 doi:http://dx.doi.org/10.1002/pro.3053
|
