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| | <StructureSection load='5ks9' size='340' side='right'caption='[[5ks9]], [[Resolution|resolution]] 2.55Å' scene=''> | | <StructureSection load='5ks9' size='340' side='right'caption='[[5ks9]], [[Resolution|resolution]] 2.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ks9]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KS9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KS9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ks9]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KS9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HLA-DQA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), HLA-DQB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4565 Canadian hard winter wheat])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ks9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ks9 OCA], [http://pdbe.org/5ks9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ks9 RCSB], [http://www.ebi.ac.uk/pdbsum/5ks9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ks9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ks9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ks9 OCA], [https://pdbe.org/5ks9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ks9 RCSB], [https://www.ebi.ac.uk/pdbsum/5ks9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ks9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/L8E864_HUMAN L8E864_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[T-cell receptor|T-cell receptor]] | + | *[[T-cell receptor 3D structures|T-cell receptor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Canadian hard winter wheat]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Petersen, J]] | + | [[Category: Triticum aestivum]] |
| - | [[Category: Reid, H H]] | + | [[Category: Petersen J]] |
| - | [[Category: Rossjohn, J]]
| + | [[Category: Reid HH]] |
| - | [[Category: Celiac disease t cell receptor peptide mhc complex]] | + | [[Category: Rossjohn J]] |
| - | [[Category: Immune system]] | + | |
| Structural highlights
Function
L8E864_HUMAN
Publication Abstract from PubMed
In HLA-DQ8-associated celiac disease, TRAV26-2+-TRBV9+ and TRAV8-3+-TRBV6+ T cells recognize the immunodominant DQ8-glia-alpha1 epitope, whereupon a non-germline-encoded arginine residue played a key role in binding HLA-DQ8-glia-alpha1. Whether distinct T cell receptor (TCR) recognition modes exist for gliadin epitopes remains unclear. TCR repertoire analysis revealed populations of HLA-DQ8-glia-alpha1 and HLA-DQ8.5-glia-gamma1 restricted TRAV20+-TRBV9+ T cells that did not possess a non-germline-encoded arginine residue. The crystal structures of a TRAV20+-TRBV9+ TCR-HLA-DQ8-glia-alpha1 complex and two TRAV20+-TRBV9+ TCR-HLA-DQ8.5-glia-gamma1 complexes were determined. This revealed that the differential specificity toward DQ8-glia-alpha1 and DQ8.5-glia-gamma1 was governed by CDR3beta-loop-mediated interactions. Surprisingly, a germline-encoded arginine residue within the CDR1alpha loop of the TRAV20+ TCR substituted for the role of the non-germline-encoded arginine in the TRAV26-2+-TRBV9+ and TRAV8-3+-TRBV6+ TCRs. Thus, in celiac disease, the responding TCR repertoire is driven by a common mechanism that selects for structural elements within the TCR that have convergent binding solutions in HLA-DQ8-gliadin recognition.
Diverse T Cell Receptor Gene Usage in HLA-DQ8-Associated Celiac Disease Converges into a Consensus Binding Solution.,Petersen J, Kooy-Winkelaar Y, Loh KL, Tran M, van Bergen J, Koning F, Rossjohn J, Reid HH Structure. 2016 Aug 23. pii: S0969-2126(16)30221-0. doi:, 10.1016/j.str.2016.07.010. PMID:27568928[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Petersen J, Kooy-Winkelaar Y, Loh KL, Tran M, van Bergen J, Koning F, Rossjohn J, Reid HH. Diverse T Cell Receptor Gene Usage in HLA-DQ8-Associated Celiac Disease Converges into a Consensus Binding Solution. Structure. 2016 Aug 23. pii: S0969-2126(16)30221-0. doi:, 10.1016/j.str.2016.07.010. PMID:27568928 doi:http://dx.doi.org/10.1016/j.str.2016.07.010
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