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| | <StructureSection load='5vyo' size='340' side='right'caption='[[5vyo]], [[Resolution|resolution]] 2.49Å' scene=''> | | <StructureSection load='5vyo' size='340' side='right'caption='[[5vyo]], [[Resolution|resolution]] 2.49Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5vyo]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VYO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5vyo]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei] and [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VYO FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dsbA, BPSL0381 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272560 BURPS])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vyo OCA], [http://pdbe.org/5vyo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vyo RCSB], [http://www.ebi.ac.uk/pdbsum/5vyo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vyo ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vyo OCA], [https://pdbe.org/5vyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vyo RCSB], [https://www.ebi.ac.uk/pdbsum/5vyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vyo ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DSBB_BURPS DSBB_BURPS]] Required for disulfide bond formation in some periplasmic proteins. Acts by oxidizing the DsbA protein. | + | [https://www.uniprot.org/uniprot/Q63Y08_BURPS Q63Y08_BURPS] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Burps]] | + | [[Category: Burkholderia pseudomallei]] |
| | + | [[Category: Burkholderia pseudomallei K96243]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Martin, J L]] | + | [[Category: Martin JL]] |
| - | [[Category: McMahon, R M]] | + | [[Category: McMahon RM]] |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Thioredoxin]]
| + | |
| Structural highlights
Function
Q63Y08_BURPS
Publication Abstract from PubMed
The naturally antibiotic-resistant bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a disease with stubbornly high mortality and a complex, protracted treatment regimen. The worldwide incidence of melioidosis is likely grossly underreported, though it is known to be highly endemic in northern Australia and Southeast Asia. Bacterial disulfide bond proteins (DSB) catalyze the oxidative folding and isomerization of disulfide bonds in substrate proteins. In the present study, we demonstrate that B. pseudomallei membrane protein disulfide bond protein B (BpsDsbB) forms a functional redox relay with the previously characterized virulence mediator disulfide bond protein A (BpsDsbA). Genomic analysis of diverse B. pseudomallei clinical isolates demonstrated that dsbB is a highly conserved core gene. Critically, we show that DsbB is required for virulence in B. pseudomallei A panel of B. pseudomalleidsbB deletion strains (K96243, 576, MSHR2511, MSHR0305b and MSHR5858) were phenotypically diverse with in vitro assays that assess hallmarks of virulence. Irrespective of in vitro virulence phenotypes, two deletion strains were attenuated in a BALB/c mouse model of infection. A crystal structure of a DsbB-derived peptide complexed with BpsDsbA provides the first molecular characterization of their interaction. This work contributes to our broader understanding of DSB redox biology, and will support antimicrobial drug design against this important family of bacterial virulence targets.
Virulence of the melioidosis pathogen Burkholderia pseudomallei requires the oxidoreductase membrane protein DsbB.,McMahon RM, Ireland PM, Sarovich DS, Petit G, Jenkins CH, Sarkar-Tyson M, Currie BJ, Martin JL Infect Immun. 2018 Feb 12. pii: IAI.00938-17. doi: 10.1128/IAI.00938-17. PMID:29440370[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ McMahon RM, Ireland PM, Sarovich DS, Petit G, Jenkins CH, Sarkar-Tyson M, Currie BJ, Martin JL. Virulence of the melioidosis pathogen Burkholderia pseudomallei requires the oxidoreductase membrane protein DsbB. Infect Immun. 2018 Feb 12. pii: IAI.00938-17. doi: 10.1128/IAI.00938-17. PMID:29440370 doi:http://dx.doi.org/10.1128/IAI.00938-17
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