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6as4

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Structure of a phage anti-CRISPR protein

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Retrieved from "http://52.214.119.220/wiki/index.php/6as4"

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 <StructureSection load='6as4' size='340' side='right'caption='[[6as4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6as4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_jbd5 Pseudomonas phage jbd5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AS4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6as4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_JBD5 Pseudomonas phage JBD5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AS4 FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">JBD5_034 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223261 Pseudomonas phage JBD5])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6as4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6as4 OCA], [http://pdbe.org/6as4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6as4 RCSB], [http://www.ebi.ac.uk/pdbsum/6as4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6as4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6as4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6as4 OCA], [https://pdbe.org/6as4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6as4 RCSB], [https://www.ebi.ac.uk/pdbsum/6as4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6as4 ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/L7P7L6_9CAUD L7P7L6_9CAUD]
-CRISPR (clustered regularly interspaced short palindromic repeat)-Cas adaptive immune systems are prevalent defense mechanisms in bacteria and archaea. They provide sequence-specific detection and neutralization of foreign nucleic acids such as bacteriophages and plasmids. One mechanism by which phages and other mobile genetic elements are able to overcome the CRISPR-Cas system is through the expression of anti-CRISPR proteins. Over 20 different families of anti-CRISPR proteins have been described, each of which inhibits a particular type of CRISPR-Cas system. In this work, we determined the structure of type I-E anti-CRISPR protein AcrE1 by X-ray crystallography. We show that AcrE1 binds to the CRISPR-associated helicase/nuclease Cas3 and that the C-terminal region of the anti-CRISPR protein is important for its inhibitory activity. We further show that AcrE1 can convert the endogenous type I-E CRISPR system into a programmable transcriptional repressor.IMPORTANCE The CRISPR-Cas immune system provides bacteria with resistance to invasion by potentially harmful viruses, plasmids, and other foreign mobile genetic elements. This study presents the first structural and mechanistic insight into a phage-encoded protein that inactivates the type I-E CRISPR-Cas system in Pseudomonas aeruginosa The interaction of this anti-CRISPR protein with the CRISPR-associated helicase/nuclease proteins Cas3 shuts down the CRISPR-Cas system and protects phages carrying this gene from destruction. This interaction also allows the repurposing of the endogenous type I-E CRISPR system into a programmable transcriptional repressor, providing a new biotechnological tool for genetic studies of bacteria encoding this type I-E CRISPR-Cas system.
-Disabling a Type I-E CRISPR-Cas Nuclease with a Bacteriophage-Encoded Anti-CRISPR Protein.,Pawluk A, Shah M, Mejdani M, Calmettes C, Moraes TF, Davidson AR, Maxwell KL MBio. 2017 Dec 12;8(6). pii: mBio.01751-17. doi: 10.1128/mBio.01751-17. PMID:29233895<ref>PMID:29233895</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 6as4" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Pseudomonas phage jbd5]]
+[[Category: Pseudomonas phage JBD5]]
-[[Category: Calmettes, C]]
+[[Category: Calmettes C]]
-[[Category: Davidson, A R]]
+[[Category: Davidson AR]]
-[[Category: Maxwell, K L]]
+[[Category: Maxwell KL]]
-[[Category: Mejdani, M]]
+[[Category: Mejdani M]]
-[[Category: Moraes, T F]]
+[[Category: Moraes TF]]
-[[Category: Pawluk, A]]
+[[Category: Pawluk A]]
-[[Category: Shah, M]]
+[[Category: Shah M]]
-[[Category: Phage protein]]
-[[Category: Viral protein]]

6as4

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Structure of a phage anti-CRISPR protein

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