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| | ==Solution NMR structures for CcoTx-II== | | ==Solution NMR structures for CcoTx-II== |
| - | <StructureSection load='6btv' size='340' side='right'caption='[[6btv]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6btv' size='340' side='right'caption='[[6btv]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6btv]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BTV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BTV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6btv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ceratogyrus_marshalli Ceratogyrus marshalli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BTV FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6br0|6br0]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6btv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6btv OCA], [https://pdbe.org/6btv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6btv RCSB], [https://www.ebi.ac.uk/pdbsum/6btv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6btv ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6btv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6btv OCA], [http://pdbe.org/6btv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6btv RCSB], [http://www.ebi.ac.uk/pdbsum/6btv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6btv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TX2_CERMR TX2_CERMR]] Inhibits several voltage-gated sodium channels and only one voltage-gated calcium channel (Cav2.2/CACNA1B (IC(50)=1100 nM) and Nav1.2/SCN2A (IC(50)=8-80 nM), Nav1.3/SCN3A (88-5570 nM), Nav1.1/SCN1A (170-407 nM), Nav1.7/SCN9A (230 nM), Nav1.6/SCN6A (3990 nM), Nav1.4/SCN4A (400 nM or >10 uM), Nav1.5/SCN5A (1634 nM or >10 uM)) (PubMed:16267209, PubMed:28880874). The toxin acts by shifting the voltage dependence of channel activation to more depolarized potentials and by blocking the inward component of the sodium current (PubMed:16267209). In vivo, this toxin causes general ataxia, lack of response to stimuli, and semiparalysis (PubMed:16267209). After a few minutes, the mice are unable to stand, and breathing is reduced in rhythm and intensity (PubMed:16267209). Symptoms gradually increase with progressive slowing of breathing and flaccid paralysis; death occurred within 10 to 20 minutes post injection (PubMed:16267209). Animals remain totally flaccid, and no symptoms of excitatory neurotoxicity are observed (PubMed:16267209).<ref>PMID:16267209</ref> <ref>PMID:28880874</ref> | + | [https://www.uniprot.org/uniprot/TX2_CERMR TX2_CERMR] Inhibits several voltage-gated sodium channels and only one voltage-gated calcium channel (Cav2.2/CACNA1B (IC(50)=1100 nM) and Nav1.2/SCN2A (IC(50)=8-80 nM), Nav1.3/SCN3A (88-5570 nM), Nav1.1/SCN1A (170-407 nM), Nav1.7/SCN9A (230 nM), Nav1.6/SCN6A (3990 nM), Nav1.4/SCN4A (400 nM or >10 uM), Nav1.5/SCN5A (1634 nM or >10 uM)) (PubMed:16267209, PubMed:28880874). The toxin acts by shifting the voltage dependence of channel activation to more depolarized potentials and by blocking the inward component of the sodium current (PubMed:16267209). In vivo, this toxin causes general ataxia, lack of response to stimuli, and semiparalysis (PubMed:16267209). After a few minutes, the mice are unable to stand, and breathing is reduced in rhythm and intensity (PubMed:16267209). Symptoms gradually increase with progressive slowing of breathing and flaccid paralysis; death occurred within 10 to 20 minutes post injection (PubMed:16267209). Animals remain totally flaccid, and no symptoms of excitatory neurotoxicity are observed (PubMed:16267209).<ref>PMID:16267209</ref> <ref>PMID:28880874</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Ceratogyrus marshalli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Agwa, A J]] | + | [[Category: Agwa AJ]] |
| - | [[Category: Schroeder, C I]] | + | [[Category: Schroeder CI]] |
| - | [[Category: Disulfide]]
| + | |
| - | [[Category: Ick]]
| + | |
| - | [[Category: Nav1 7]]
| + | |
| - | [[Category: Pain]]
| + | |
| - | [[Category: Spider]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Voltage gated ion channel]]
| + | |
| Structural highlights
Function
TX2_CERMR Inhibits several voltage-gated sodium channels and only one voltage-gated calcium channel (Cav2.2/CACNA1B (IC(50)=1100 nM) and Nav1.2/SCN2A (IC(50)=8-80 nM), Nav1.3/SCN3A (88-5570 nM), Nav1.1/SCN1A (170-407 nM), Nav1.7/SCN9A (230 nM), Nav1.6/SCN6A (3990 nM), Nav1.4/SCN4A (400 nM or >10 uM), Nav1.5/SCN5A (1634 nM or >10 uM)) (PubMed:16267209, PubMed:28880874). The toxin acts by shifting the voltage dependence of channel activation to more depolarized potentials and by blocking the inward component of the sodium current (PubMed:16267209). In vivo, this toxin causes general ataxia, lack of response to stimuli, and semiparalysis (PubMed:16267209). After a few minutes, the mice are unable to stand, and breathing is reduced in rhythm and intensity (PubMed:16267209). Symptoms gradually increase with progressive slowing of breathing and flaccid paralysis; death occurred within 10 to 20 minutes post injection (PubMed:16267209). Animals remain totally flaccid, and no symptoms of excitatory neurotoxicity are observed (PubMed:16267209).[1] [2]
Publication Abstract from PubMed
Gating modifier toxins (GMTs) are venom-derived peptides isolated from spiders and other venomous creatures that modulate activity of disease-relevant voltage-gated ion channels and are therefore being pursued as therapeutic leads. The amphipathic surface profile of GMTs has prompted the proposal that some GMTs simultaneously bind to the cell membrane and voltage-gated ion channels in a trimolecular complex. Here we examined whether there is a relationship among spider GMT amphipathicity, membrane binding and potency or selectivity for voltage-gated sodium (NaV) channels. We used NMR spectroscopy and in silico calculations to examine the structures and physicochemical properties of a panel of nine GMTs and deployed surface plasmon resonance to measure GMT affinity for lipids putatively found in proximity to NaV channels. Electrophysiology was used to quantify GMT activity on NaV1.7, an ion channel linked to chronic pain. Selectivity of the peptides was further examined against a panel of NaV channel subtypes. We show that GMTs adsorb to the outer leaflet of anionic lipid bilayers through electrostatic interactions. We did not observe a direct correlation between GMT amphipathicity and affinity for lipid bilayers. Furthermore, GMT-lipid bilayer interactions did not correlate with potency or selectivity for NaVs. We therefore propose that increased membrane binding is unlikely to improve subtype selectivity and that the conserved amphipathic GMT surface profile is an adaptation that facilitates simultaneous modulation of multiple NaVs.
Gating modifier toxins isolated from spider venom: modulation of voltage-gated sodium channels and the role of lipid membranes.,Agwa AJ, Peigneur S, Chow CY, Lawrence N, Craik DJ, Tytgat J, King GF, Henriques ST, Schroeder CI J Biol Chem. 2018 Apr 27. pii: RA118.002553. doi: 10.1074/jbc.RA118.002553. PMID:29703751[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bosmans F, Rash L, Zhu S, Diochot S, Lazdunski M, Escoubas P, Tytgat J. Four novel tarantula toxins as selective modulators of voltage-gated sodium channel subtypes. Mol Pharmacol. 2006 Feb;69(2):419-29. Epub 2005 Nov 2. PMID:16267209 doi:http://dx.doi.org/mol.105.015941
- ↑ Sousa SR, Wingerd JS, Brust A, Bladen C, Ragnarsson L, Herzig V, Deuis JR, Dutertre S, Vetter I, Zamponi GW, King GF, Alewood PF, Lewis RJ. Discovery and mode of action of a novel analgesic beta-toxin from the African spider Ceratogyrus darlingi. PLoS One. 2017 Sep 7;12(9):e0182848. doi: 10.1371/journal.pone.0182848., eCollection 2017. PMID:28880874 doi:http://dx.doi.org/10.1371/journal.pone.0182848
- ↑ Agwa AJ, Peigneur S, Chow CY, Lawrence N, Craik DJ, Tytgat J, King GF, Henriques ST, Schroeder CI. Gating modifier toxins isolated from spider venom: modulation of voltage-gated sodium channels and the role of lipid membranes. J Biol Chem. 2018 Apr 27. pii: RA118.002553. doi: 10.1074/jbc.RA118.002553. PMID:29703751 doi:http://dx.doi.org/10.1074/jbc.RA118.002553
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