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6uug

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Revision as of 06:38, 6 May 2020

Structure of methanesulfinate monooxygenase MsuC from Pseudomonas fluorescens at 1.69 angstrom resolution

Structural highlights

6uug is a 2 chain structure with sequence from Psepf. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	6u76
Gene:	Pfl01_3917 (PSEPF)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Methyl sulfur compounds are a rich source of environmental sulfur for microorganisms, but their use requires redox systems. The bacterial sfn and msu operons contain two-component flavin-dependent monooxygenases for dimethylsulfone (DMSO2) assimilation: SfnG converts DMSO2 to methanesulfinate (MSI(-)), and MsuD converts methanesulfonate (MS(-)) to sulfite. However, the enzymatic oxidation of MSI(-) to MS(-) has not been demonstrated, and the function of the last enzyme of the msu operon (MsuC) is unresolved. We employed crystallographic and biochemical studies to identify the function of MsuC from Pseudomonas fluorescens. The crystal structure of MsuC adopts the acyl-CoA dehydrogenase fold with putative binding sites for flavin and MSI(-), and functional assays of MsuC in the presence of its oxidoreductase MsuE, FMN, and NADH confirm the enzymatic generation of MS(-). These studies reveal that MsuC converts MSI(-) to MS(-) in sulfite biosynthesis from DMSO2.

Structure and function of the two-component flavin-dependent methanesulfinate monooxygenase within bacterial sulfur assimilation.,Soule J, Gnann AD, Gonzalez R, Parker MJ, McKenna KC, Nguyen SV, Phan NT, Wicht DK, Dowling DP Biochem Biophys Res Commun. 2019 Nov 18. pii: S0006-291X(19)32128-X. doi:, 10.1016/j.bbrc.2019.11.008. PMID:31753487^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Soule J, Gnann AD, Gonzalez R, Parker MJ, McKenna KC, Nguyen SV, Phan NT, Wicht DK, Dowling DP. Structure and function of the two-component flavin-dependent methanesulfinate monooxygenase within bacterial sulfur assimilation. Biochem Biophys Res Commun. 2019 Nov 18. pii: S0006-291X(19)32128-X. doi:, 10.1016/j.bbrc.2019.11.008. PMID:31753487 doi:http://dx.doi.org/10.1016/j.bbrc.2019.11.008

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6uug"

@@ Line 3: / Line 3: @@
 <StructureSection load='6uug' size='340' side='right'caption='[[6uug]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6uug]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psepf Psepf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UUG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6uug]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Psepf Psepf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UUG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UUG FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6u76|6u76]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Pfl01_3917 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=205922 PSEPF])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uug OCA], [http://pdbe.org/6uug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uug RCSB], [http://www.ebi.ac.uk/pdbsum/6uug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uug ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uug OCA], [http://pdbe.org/6uug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uug RCSB], [http://www.ebi.ac.uk/pdbsum/6uug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uug ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 24: / Line 24: @@
 [[Category: Psepf]]
 [[Category: Dowling, D P]]
-[[Category: Gnann, A G]]
+[[Category: Gnann, A D]]
 [[Category: Gonzalez, R]]
 [[Category: McKenna, K C]]

6uug

From Proteopedia

Revision as of 06:38, 6 May 2020

Structure of methanesulfinate monooxygenase MsuC from Pseudomonas fluorescens at 1.69 angstrom resolution

Structural highlights

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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