1vb0

From Proteopedia

(Difference between revisions)

Revision as of 13:19, 13 October 2021

Atomic resolution structure of atratoxin-b, one short-chain neurotoxin from Naja atra

Structural highlights

1vb0 is a 1 chain structure with sequence from Naja atra. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[3S1CC_NAJAT] Binds to muscle nicotinic acetylcholine receptor (nAChR) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Has a lower toxicity than cobrotoxin.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

By using single wavelength anomalous diffraction phasing based on the anomalous signal from copper atoms, the crystal structure of atratoxin was determined at the resolution of 1.5 A and was refined to an ultrahigh resolution of 0.87 A. The ultrahigh resolution electron density maps allowed the modeling of 38 amino acid residues in alternate conformations and the location of 322 of 870 possible hydrogen atoms. To get accurate information at the atomic level, atratoxin-b (an analog of atratoxin with reduced toxicity) was also refined to an atomic resolution of 0.92 A. By the sequence and structural comparison of these two atratoxins, Arg(33) and Arg(36) were identified to be critical to their varied toxicity. The effect of copper ions on the distribution of hydrogen atoms in atratoxin was discussed, and the interactions between copper ions and protein residues were analyzed based on a statistical method, revealing a novel pentahedral copper-binding motif.

The atomic resolution crystal structure of atratoxin determined by single wavelength anomalous diffraction phasing.,Lou X, Liu Q, Tu X, Wang J, Teng M, Niu L, Schuller DJ, Huang Q, Hao Q J Biol Chem. 2004 Sep 10;279(37):39094-104. Epub 2004 Jul 12. PMID:15252034^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chang LS, Chou YC, Lin SR, Wu BN, Lin J, Hong E, Sun YJ, Hsiao CD. A novel neurotoxin, cobrotoxin b, from Naja naja atra (Taiwan cobra) venom: purification, characterization, and gene organization. J Biochem. 1997 Dec;122(6):1252-9. PMID:9498573
↑ Lou X, Liu Q, Tu X, Wang J, Teng M, Niu L, Schuller DJ, Huang Q, Hao Q. The atomic resolution crystal structure of atratoxin determined by single wavelength anomalous diffraction phasing. J Biol Chem. 2004 Sep 10;279(37):39094-104. Epub 2004 Jul 12. PMID:15252034 doi:10.1074/jbc.M403863200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1vb0"

@@ Line 3: / Line 3: @@
 <StructureSection load='1vb0' size='340' side='right'caption='[[1vb0]], [[Resolution|resolution]] 0.92&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1vb0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VB0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VB0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1vb0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VB0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vb0 OCA], [http://pdbe.org/1vb0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vb0 RCSB], [http://www.ebi.ac.uk/pdbsum/1vb0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1vb0 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vb0 OCA], [https://pdbe.org/1vb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vb0 RCSB], [https://www.ebi.ac.uk/pdbsum/1vb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vb0 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/3S1CC_NAJAT 3S1CC_NAJAT]] Binds to muscle nicotinic acetylcholine receptor (nAChR) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Has a lower toxicity than cobrotoxin.<ref>PMID:9498573</ref>
+[[https://www.uniprot.org/uniprot/3S1CC_NAJAT 3S1CC_NAJAT]] Binds to muscle nicotinic acetylcholine receptor (nAChR) and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Has a lower toxicity than cobrotoxin.<ref>PMID:9498573</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1vb0

From Proteopedia

Revision as of 13:19, 13 October 2021

Atomic resolution structure of atratoxin-b, one short-chain neurotoxin from Naja atra

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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