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| - | [[Image:1abt.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1abt| PDB=1abt | SCENE= }} | | {{STRUCTURE_1abt| PDB=1abt | SCENE= }} |
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| - | '''NMR SOLUTION STRUCTURE OF AN ALPHA-BUNGAROTOXIN(SLASH)NICOTINIC RECEPTOR PEPTIDE COMPLEX'''
| + | ===NMR SOLUTION STRUCTURE OF AN ALPHA-BUNGAROTOXIN(SLASH)NICOTINIC RECEPTOR PEPTIDE COMPLEX=== |
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| - | ==Overview==
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| - | We report the two-dimensional nuclear magnetic resonance (NMR) characterization of the stoichiometric complex formed between the snake venom-derived long alpha-neurotoxin, alpha-bungarotoxin (BGTX), and a synthetic dodecapeptide (alpha 185-196) corresponding to a functionally important region on the alpha-subunit of the nicotinic acetylcholine receptor (nAChR) obtained from Torpedo californica electric organ tissue. BGTX has been widely used as the classic nicotinic competitive antagonist for the skeletal muscle type of nAChR which is found in the avian, amphibian, and mammalian neuromuscular junction. The receptor dodecapeptide (alpha 185-196) binds BGTX with micromolar affinity and has been shown to represent the major determinant of BGTX binding to the isolated alpha-subunit. Previous studies involving covalent modification of the native nAChR from Torpedo membranes with a variety of affinity reagents indicate that several residues contained within the dodecapeptide sequence (namely, Tyr-190, Cys-192, and Cys-193) apparently contribute directly to the formation of the cholinergic ligand binding site. The NMR-derived solution structure of the BGTX/receptor peptide complex defines a relatively extended conformation for a major segment of the "bound" dodecapeptide. These structural studies also reveal a previously unpredicted receptor binding cleft within BGTX and suggest that BGTX undergoes a conformational change upon peptide binding. If, as we hypothesize, the identified intermolecular contacts in the BGTX/receptor peptide complex describe a portion of the contact zone between BGTX and native receptor, then the structural data would suggest that alpha-subunit residues 186-190 are on the extracellular surface of the receptor.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_8241115}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8241115 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8241115}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1ABT is a [[Protein complex]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ABT OCA]. | + | 1ABT is a [[Protein complex]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ABT OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Song, G.]] | | [[Category: Song, G.]] |
| | [[Category: Toxin]] | | [[Category: Toxin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:04:59 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:32:54 2008'' |
Revision as of 13:32, 30 June 2008
Template:STRUCTURE 1abt
NMR SOLUTION STRUCTURE OF AN ALPHA-BUNGAROTOXIN(SLASH)NICOTINIC RECEPTOR PEPTIDE COMPLEX
Template:ABSTRACT PUBMED 8241115
About this Structure
1ABT is a Protein complex structure. Full experimental information is available from OCA.
Reference
NMR solution structure of an alpha-bungarotoxin/nicotinic receptor peptide complex., Basus VJ, Song G, Hawrot E, Biochemistry. 1993 Nov 23;32(46):12290-8. PMID:8241115
Page seeded by OCA on Mon Jun 30 16:32:54 2008
