6kza

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Current revision (10:44, 27 March 2024) (edit) (undo)
 
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<StructureSection load='6kza' size='340' side='right'caption='[[6kza]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
<StructureSection load='6kza' size='340' side='right'caption='[[6kza]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6kza]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KZA FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6kza]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KZA FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnaB, EVY21_06600 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), dnaC, EYX85_09815, EYY00_08890, EYY22_09440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kza OCA], [https://pdbe.org/6kza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kza RCSB], [https://www.ebi.ac.uk/pdbsum/6kza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kza ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kza OCA], [http://pdbe.org/6kza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kza RCSB], [http://www.ebi.ac.uk/pdbsum/6kza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kza ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/DNAB_ECOLI DNAB_ECOLI] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.
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Loading the bacterial replicative helicase DnaB onto DNA requires a specific loader protein, DnaC/DnaI, which creates the loading-competent state by opening the DnaB hexameric ring. To understand the molecular mechanism by which DnaC/DnaI opens the DnaB ring, we solved 3.1-A co-crystal structure of the interaction domains of Escherichia coli DnaB-DnaC. The structure reveals that one N-terminal domain (NTD) of DnaC interacts with both the linker helix of a DnaB molecule and the C-terminal domain (CTD) of the adjacent DnaB molecule by forming a three alpha-helix bundle, which fixes the relative orientation of the two adjacent DnaB CTDs. The importance of the intermolecular interface in the crystal structure was supported by the mutational data of DnaB and DnaC. Based on the crystal structure and other available information on DnaB-DnaC structures, we constructed a molecular model of the hexameric DnaB CTDs bound by six DnaC NTDs. This model suggested that the binding of a DnaC would cause a distortion in the hexameric ring of DnaB. This distortion of the DnaB ring might accumulate by the binding of up to six DnaC molecules, resulting in the DnaB ring to open.
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Crystal structure of the complex of the interaction domains of E. coli DnaB helicase and DnaC helicase loader: Structural basis implying a distortion-accumulation mechanism for the DnaB ring opening caused by DnaC binding.,Nagata K, Okada A, Ohtsuka J, Ohkuri T, Akama Y, Sakiyama Y, Miyazaki E, Horita S, Katayama T, Ueda T, Tanokura M J Biochem. 2019 Oct 30. pii: 5610074. doi: 10.1093/jb/mvz087. PMID:31665315<ref>PMID:31665315</ref>
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==See Also==
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*[[DnaC helicase loader|DnaC helicase loader]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[Helicase 3D structures|Helicase 3D structures]]
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</div>
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<div class="pdbe-citations 6kza" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus coli migula 1895]]
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[[Category: Escherichia coli]]
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[[Category: DNA helicase]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Akama, Y]]
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[[Category: Akama Y]]
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[[Category: Horita, S]]
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[[Category: Horita S]]
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[[Category: Katayama, T]]
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[[Category: Katayama T]]
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[[Category: Miyazaki, E]]
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[[Category: Miyazaki E]]
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[[Category: Nagata, K]]
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[[Category: Nagata K]]
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[[Category: Ohkuri, T]]
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[[Category: Ohkuri T]]
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[[Category: Ohtsuka, J]]
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[[Category: Ohtsuka J]]
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[[Category: Okada, A]]
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[[Category: Okada A]]
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[[Category: Sakiyama, Y]]
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[[Category: Sakiyama Y]]
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[[Category: Tanokura, M]]
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[[Category: Tanokura M]]
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[[Category: Ueda, T]]
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[[Category: Ueda T]]
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[[Category: Dnab]]
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[[Category: Dnac]]
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[[Category: Helicase]]
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[[Category: Helicase loader protein]]
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[[Category: Hydrolase]]
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[[Category: Replication]]
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[[Category: Structural genomic]]
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Current revision

Crystal structure of the complex of the interaction domains of E. coli DnaB helicase and DnaC helicase loader

PDB ID 6kza

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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