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| - | [[Image:1afb.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1afb| PDB=1afb | SCENE= }} | | {{STRUCTURE_1afb| PDB=1afb | SCENE= }} |
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| - | '''STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS'''
| + | ===STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS=== |
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| - | ==Overview==
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| - | The asialoglycoprotein receptors and many other C-type (Ca2+-dependent) animal lectins specifically recognize galactose- or N-acetylgalactosamine-terminated oligosaccharides. Analogous binding specificity can be engineered into the homologous rat mannose-binding protein A by changing three amino acids and inserting a glycine-rich loop (Iobst, S. T., and Drickamer, K. (1994) J. Biol. Chem. 269, 15512-15519). Crystal structures of this mutant complexed with beta-methyl galactoside and N-acetylgalactosamine (GalNAc) reveal that as with wild-type mannose-binding proteins, the 3- and 4-OH groups of the sugar directly coordinate Ca2+ and form hydrogen bonds with amino acids that also serve as Ca2+ ligands. The different stereochemistry of the 3- and 4-OH groups in mannose and galactose, combined with a fixed Ca2+ coordination geometry, leads to different pyranose ring locations in the two cases. The glycine-rich loop provides selectivity against mannose by holding a critical tryptophan in a position optimal for packing with the apolar face of galactose but incompatible with mannose binding. The 2-acetamido substituent of GalNAc is in the vicinity of amino acid positions identified by site-directed mutagenesis (Iobst, S. T., and Drickamer, K. (1996) J. Biol. Chem. 271, 6686-6693) as being important for the formation of a GalNAc-selective binding site. | + | The line below this paragraph, {{ABSTRACT_PUBMED_8636086}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8636086 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8636086}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: C-type lectin]] | | [[Category: C-type lectin]] |
| | [[Category: Calcium-binding protein]] | | [[Category: Calcium-binding protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:11:38 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:43:43 2008'' |
Revision as of 13:43, 30 June 2008
Template:STRUCTURE 1afb
STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS
Template:ABSTRACT PUBMED 8636086
About this Structure
1AFB is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structural basis of galactose recognition by C-type animal lectins., Kolatkar AR, Weis WI, J Biol Chem. 1996 Mar 22;271(12):6679-85. PMID:8636086
Page seeded by OCA on Mon Jun 30 16:43:43 2008
