We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1afd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1afd.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1afd.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1afd| PDB=1afd | SCENE= }}
{{STRUCTURE_1afd| PDB=1afd | SCENE= }}
-
'''STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS'''
+
===STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS===
-
==Overview==
+
<!--
-
The asialoglycoprotein receptors and many other C-type (Ca2+-dependent) animal lectins specifically recognize galactose- or N-acetylgalactosamine-terminated oligosaccharides. Analogous binding specificity can be engineered into the homologous rat mannose-binding protein A by changing three amino acids and inserting a glycine-rich loop (Iobst, S. T., and Drickamer, K. (1994) J. Biol. Chem. 269, 15512-15519). Crystal structures of this mutant complexed with beta-methyl galactoside and N-acetylgalactosamine (GalNAc) reveal that as with wild-type mannose-binding proteins, the 3- and 4-OH groups of the sugar directly coordinate Ca2+ and form hydrogen bonds with amino acids that also serve as Ca2+ ligands. The different stereochemistry of the 3- and 4-OH groups in mannose and galactose, combined with a fixed Ca2+ coordination geometry, leads to different pyranose ring locations in the two cases. The glycine-rich loop provides selectivity against mannose by holding a critical tryptophan in a position optimal for packing with the apolar face of galactose but incompatible with mannose binding. The 2-acetamido substituent of GalNAc is in the vicinity of amino acid positions identified by site-directed mutagenesis (Iobst, S. T., and Drickamer, K. (1996) J. Biol. Chem. 271, 6686-6693) as being important for the formation of a GalNAc-selective binding site.
+
The line below this paragraph, {{ABSTRACT_PUBMED_8636086}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 8636086 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_8636086}}
==About this Structure==
==About this Structure==
Line 26: Line 30:
[[Category: C-type lectin]]
[[Category: C-type lectin]]
[[Category: Calcium-binding protein]]
[[Category: Calcium-binding protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:11:50 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:44:05 2008''

Revision as of 13:44, 30 June 2008

Image:1afd.png

Template:STRUCTURE 1afd

STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS

Template:ABSTRACT PUBMED 8636086

About this Structure

1AFD is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structural basis of galactose recognition by C-type animal lectins., Kolatkar AR, Weis WI, J Biol Chem. 1996 Mar 22;271(12):6679-85. PMID:8636086

Page seeded by OCA on Mon Jun 30 16:44:05 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1afd"
Personal tools