This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1afh

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1afh.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1afh.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1afh| PDB=1afh | SCENE= }}
{{STRUCTURE_1afh| PDB=1afh | SCENE= }}
-
'''LIPID TRANSFER PROTEIN FROM MAIZE SEEDLINGS, NMR, 15 STRUCTURES'''
+
===LIPID TRANSFER PROTEIN FROM MAIZE SEEDLINGS, NMR, 15 STRUCTURES===
-
==Overview==
+
<!--
-
The three-dimensional solution structure of a nonspecific lipid transfer protein extracted from maize seeds determined by 1H NMR spectroscopy is described. This cationic protein consists of 93 amino acid residues. Its structure was determined from 1,091 NOE-derived distance restraints, including 929 interresidue connectivities and 197 dihedral restraints (phi, psi, chi 1) derived from NOEs and 3J coupling constants. The global fold involving four helical fragments connected by three loops and a C-terminal tail without regular secondary structures is stabilized by four disulfide bridges. The most striking feature of this structure is the existence of an internal hydrophobic cavity running through the whole molecule. The global fold of this protein, very similar to that of a previously described lipid transfer protein extracted from wheat seeds (Gincel E et al., 1994, Eur J Biochem 226:413-422) constitutes a new architecture for alpha-class proteins. 1H NMR and fluorescence studies show that this protein forms well-defined complexes in aqueous solution with lysophosphatidylcholine. Dissociation constants, Kd, of 1.9 +/- 0.6 x 10(-6) M and &gt; 10(-3) M were obtained with lyso-C16 and -C12, respectively. A structure model for a lipid-protein complex is proposed in which the aliphatic chain of the phospholipid is inserted in the internal cavity and the polar head interacts with the charged side chains located at one end of this cavity. Our model for the lipid-protein complex is qualitatively very similar to the recently published crystal structure (Shin DH et al., 1995, Structure 3:189-199).
+
The line below this paragraph, {{ABSTRACT_PUBMED_8845747}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 8845747 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_8845747}}
==About this Structure==
==About this Structure==
-
1AFH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AFH OCA].
+
1AFH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AFH OCA].
==Reference==
==Reference==
Line 35: Line 39:
[[Category: Molecular modeling]]
[[Category: Molecular modeling]]
[[Category: Nmr]]
[[Category: Nmr]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:11:58 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 16:44:07 2008''

Revision as of 13:44, 30 June 2008

Image:1afh.png

Template:STRUCTURE 1afh

LIPID TRANSFER PROTEIN FROM MAIZE SEEDLINGS, NMR, 15 STRUCTURES

Template:ABSTRACT PUBMED 8845747

About this Structure

1AFH is a Single protein structure of sequence from Zea mays. Full experimental information is available from OCA.

Reference

Solution structure and lipid binding of a nonspecific lipid transfer protein extracted from maize seeds., Gomar J, Petit MC, Sodano P, Sy D, Marion D, Kader JC, Vovelle F, Ptak M, Protein Sci. 1996 Apr;5(4):565-77. PMID:8845747

Page seeded by OCA on Mon Jun 30 16:44:07 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1afh"
Personal tools