6xwp
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of glutamate transporter homologue GltTk in unsaturated conditions - outward-outward-inward configuration== | |
| + | <StructureSection load='6xwp' size='340' side='right'caption='[[6xwp]], [[Resolution|resolution]] 3.38Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6xwp]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6XWP FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASP:ASPARTIC+ACID'>ASP</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6xwo|6xwo]], [[6xwq|6xwq]], [[6xwr|6xwr]], [[6xwn|6xwn]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6xwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwp OCA], [http://pdbe.org/6xwp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6xwp RCSB], [http://www.ebi.ac.uk/pdbsum/6xwp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwp ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glutamate transporters are cation-coupled secondary active membrane transporters that clear the neurotransmitter L-glutamate from the synaptic cleft. These transporters are homotrimers, with each protomer functioning independently by an elevator-type mechanism, in which a mobile transport domain alternates between inward- and outward-oriented states. Using single-particle cryo-EM we have determined five structures of the glutamate transporter homologue GltTk, a Na(+)- L-aspartate symporter, embedded in lipid nanodiscs. Dependent on the substrate concentrations used, the protomers of the trimer adopt a variety of asymmetrical conformations, consistent with the independent movement. Six of the 15 resolved protomers are in a hitherto elusive state of the transport cycle in which the inward-facing transporters are loaded with Na(+) ions. These structures explain how substrate-leakage is prevented - a strict requirement for coupled transport. The belt protein of the lipid nanodiscs bends around the inward oriented protomers, suggesting that membrane deformations occur during transport. | ||
| - | + | Structural ensemble of a glutamate transporter homologue in lipid nanodisc environment.,Arkhipova V, Guskov A, Slotboom DJ Nat Commun. 2020 Feb 21;11(1):998. doi: 10.1038/s41467-020-14834-8. PMID:32081874<ref>PMID:32081874</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6xwp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Arkhipova, V]] | ||
| + | [[Category: Guskov, A]] | ||
| + | [[Category: Slotboom, D J]] | ||
| + | [[Category: Amino acid transporter]] | ||
| + | [[Category: Aspartate transport]] | ||
| + | [[Category: Glutamate transporter homologue]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Transport protein]] | ||
Revision as of 06:59, 4 March 2020
Structure of glutamate transporter homologue GltTk in unsaturated conditions - outward-outward-inward configuration
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