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4o9x

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Revision as of 07:13, 25 January 2023

Crystal Structure of TcdB2-TccC3

Structural highlights

4o9x is a 1 chain structure with sequence from Photorhabdus luminescens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8GF99_PHOLU Q8GF97_PHOLU

Publication Abstract from PubMed

Tripartite Tc toxin complexes of bacterial pathogens perforate the host membrane and translocate toxic enzymes into the host cell, including in humans. The underlying mechanism is complex but poorly understood. Here we report the first, to our knowledge, high-resolution structures of a TcA subunit in its prepore and pore state and of a complete 1.7 megadalton Tc complex. The structures reveal that, in addition to a translocation channel, TcA forms four receptor-binding sites and a neuraminidase-like region, which are important for its host specificity. pH-induced opening of the shell releases an entropic spring that drives the injection of the TcA channel into the membrane. Binding of TcB/TcC to TcA opens a gate formed by a six-bladed beta-propeller and results in a continuous protein translocation channel, whose architecture and properties suggest a novel mode of protein unfolding and translocation. Our results allow us to understand key steps of infections involving Tc toxins at the molecular level.

Mechanism of Tc toxin action revealed in molecular detail.,Meusch D, Gatsogiannis C, Efremov RG, Lang AE, Hofnagel O, Vetter IR, Aktories K, Raunser S Nature. 2014 Apr 3;508(7494):61-5. doi: 10.1038/nature13015. Epub 2014 Feb 23. PMID:24572368^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Meusch D, Gatsogiannis C, Efremov RG, Lang AE, Hofnagel O, Vetter IR, Aktories K, Raunser S. Mechanism of Tc toxin action revealed in molecular detail. Nature. 2014 Apr 3;508(7494):61-5. doi: 10.1038/nature13015. Epub 2014 Feb 23. PMID:24572368 doi:http://dx.doi.org/10.1038/nature13015

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4o9x"

@@ Line 3: / Line 3: @@
 <StructureSection load='4o9x' size='340' side='right'caption='[[4o9x]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4o9x]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O9X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O9X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4o9x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_luminescens Photorhabdus luminescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O9X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O9X FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4o9y|4o9y]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o9x OCA], [https://pdbe.org/4o9x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o9x RCSB], [https://www.ebi.ac.uk/pdbsum/4o9x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o9x ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o9x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o9x OCA], [http://pdbe.org/4o9x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o9x RCSB], [http://www.ebi.ac.uk/pdbsum/4o9x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o9x ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8GF99_PHOLU Q8GF99_PHOLU] [https://www.uniprot.org/uniprot/Q8GF97_PHOLU Q8GF97_PHOLU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Aktories, K]]
+[[Category: Photorhabdus luminescens]]
-[[Category: Efremov, R G]]
+[[Category: Aktories K]]
-[[Category: Gatsogiannis, C]]
+[[Category: Efremov RG]]
-[[Category: Hofnagel, O]]
+[[Category: Gatsogiannis C]]
-[[Category: Lang, A E]]
+[[Category: Hofnagel O]]
-[[Category: Meusch, D]]
+[[Category: Lang AE]]
-[[Category: Raunser, S]]
+[[Category: Meusch D]]
-[[Category: Vetter, I R]]
+[[Category: Raunser S]]
-[[Category: Beta sheet]]
+[[Category: Vetter IR]]
-[[Category: Cocoon]]
-[[Category: Tc toxin]]
-[[Category: Toxin]]
-[[Category: Unfolding]]

4o9x

From Proteopedia

Revision as of 07:13, 25 January 2023

Crystal Structure of TcdB2-TccC3

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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