1a3o
From Proteopedia
(New page: 200px<br /> <applet load="1a3o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a3o, resolution 1.8Å" /> '''ARTIFICIAL MUTANT (A...) |
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==Overview== | ==Overview== | ||
The structures of deoxy human haemoglobin and an artificial mutant, (Tyralpha42-->His) have been solved at 120 K. While overall agreement, between these structures and others in the PDB is very good, certain side, chains are found to be shifted, absent from the electron-density map or in, different rotamers. Non-crystallographic symmetry (NCS) is very well, obeyed in the native protein, but not around the site of the changed, residue in the mutant. NCS is also not obeyed by the water molecule, invariably found in the alpha-chain haem pocket in room-temperature, crystal structures of haemoglobin. At 120 K, this water molecule, disappears from one alpha chain in the asymmetric unit but not the other. | The structures of deoxy human haemoglobin and an artificial mutant, (Tyralpha42-->His) have been solved at 120 K. While overall agreement, between these structures and others in the PDB is very good, certain side, chains are found to be shifted, absent from the electron-density map or in, different rotamers. Non-crystallographic symmetry (NCS) is very well, obeyed in the native protein, but not around the site of the changed, residue in the mutant. NCS is also not obeyed by the water molecule, invariably found in the alpha-chain haem pocket in room-temperature, crystal structures of haemoglobin. At 120 K, this water molecule, disappears from one alpha chain in the asymmetric unit but not the other. | ||
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| + | ==Disease== | ||
| + | Known diseases associated with this structure: Erythremias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Erythremias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Erythrocytosis OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], HPFH, deletion type OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Heinz body anemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Heinz body anemias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Heinz body anemias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Hemoglobin H disease OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Hypochromic microcytic anemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Methemoglobinemias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Methemoglobinemias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Sickle cell anemia OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Thalassemia, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141850 141850]], Thalassemia-beta, dominant inclusion-body OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]], Thalassemias, alpha- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141800 141800]], Thalassemias, beta- OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=141900 141900]] | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: respiratory protein]] | [[Category: respiratory protein]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 15:55:11 2007'' |
Revision as of 13:48, 12 November 2007
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ARTIFICIAL MUTANT (ALPHA Y42H) OF DEOXY HEMOGLOBIN
Contents |
Overview
The structures of deoxy human haemoglobin and an artificial mutant, (Tyralpha42-->His) have been solved at 120 K. While overall agreement, between these structures and others in the PDB is very good, certain side, chains are found to be shifted, absent from the electron-density map or in, different rotamers. Non-crystallographic symmetry (NCS) is very well, obeyed in the native protein, but not around the site of the changed, residue in the mutant. NCS is also not obeyed by the water molecule, invariably found in the alpha-chain haem pocket in room-temperature, crystal structures of haemoglobin. At 120 K, this water molecule, disappears from one alpha chain in the asymmetric unit but not the other.
Disease
Known diseases associated with this structure: Erythremias, alpha- OMIM:[141800], Erythremias, beta- OMIM:[141900], Erythrocytosis OMIM:[141850], HPFH, deletion type OMIM:[141900], Heinz body anemia OMIM:[141850], Heinz body anemias, alpha- OMIM:[141800], Heinz body anemias, beta- OMIM:[141900], Hemoglobin H disease OMIM:[141850], Hypochromic microcytic anemia OMIM:[141850], Methemoglobinemias, alpha- OMIM:[141800], Methemoglobinemias, beta- OMIM:[141900], Sickle cell anemia OMIM:[141900], Thalassemia, alpha- OMIM:[141850], Thalassemia-beta, dominant inclusion-body OMIM:[141900], Thalassemias, alpha- OMIM:[141800], Thalassemias, beta- OMIM:[141900]
About this Structure
1A3O is a Protein complex structure of sequences from Homo sapiens with HEM as ligand. Full crystallographic information is available from OCA.
Reference
The structures of deoxy human haemoglobin and the mutant Hb Tyralpha42His at 120 K., Tame JR, Vallone B, Acta Crystallogr D Biol Crystallogr. 2000 Jul;56(Pt 7):805-11. PMID:10930827
Page seeded by OCA on Mon Nov 12 15:55:11 2007
