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| | <StructureSection load='5fg3' size='340' side='right'caption='[[5fg3]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='5fg3' size='340' side='right'caption='[[5fg3]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5fg3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aerpe Aerpe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FG3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FG3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5fg3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix_K1 Aeropyrum pernix K1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FG3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FG3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">infB, APE_2374 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272557 AERPE])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fg3 OCA], [http://pdbe.org/5fg3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fg3 RCSB], [http://www.ebi.ac.uk/pdbsum/5fg3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fg3 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fg3 OCA], [https://pdbe.org/5fg3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fg3 RCSB], [https://www.ebi.ac.uk/pdbsum/5fg3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fg3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IF2P_AERPE IF2P_AERPE]] Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity). | + | [https://www.uniprot.org/uniprot/IF2P_AERPE IF2P_AERPE] Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aerpe]] | + | [[Category: Aeropyrum pernix K1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ito, K]] | + | [[Category: Ito K]] |
| - | [[Category: Miyoshi, T]] | + | [[Category: Miyoshi T]] |
| - | [[Category: Murakami, R]] | + | [[Category: Murakami R]] |
| - | [[Category: Uchiumi, T]] | + | [[Category: Uchiumi T]] |
| - | [[Category: Crenarchaea]]
| + | |
| - | [[Category: Gtpase]]
| + | |
| - | [[Category: Ribosomal subunit joining]]
| + | |
| - | [[Category: Translation]]
| + | |
| - | [[Category: Translation initiaton factor]]
| + | |
| Structural highlights
Function
IF2P_AERPE Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).
Publication Abstract from PubMed
Initiation factor 5B (IF5B) is a universally conserved translational GTPase that catalyzes ribosomal subunit joining. In eukaryotes, IF5B directly interacts via a groove in its domain IV with initiation factor 1A (IF1A), another universally conserved initiation factor, to accomplish efficient subunit joining. Here, we have determined the first structure of a crenarchaeal IF5B, which revealed that the archaea-specific region of IF5B (helix alpha15) binds and occludes the groove of domain IV. Therefore, archaeal IF5B cannot access IF1A in the same manner as eukaryotic IF5B. This fact suggests that different relationships between IF5B and IF1A exist in archaea and eukaryotes. Proteins 2016; 84:712-717. (c) 2016 Wiley Periodicals, Inc.
Crystal structure of translation initiation factor 5B from the crenarchaeon Aeropyrum pernix.,Murakami R, Miyoshi T, Uchiumi T, Ito K Proteins. 2016 May;84(5):712-7. doi: 10.1002/prot.25009. Epub 2016 Mar 6. PMID:26868175[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Murakami R, Miyoshi T, Uchiumi T, Ito K. Crystal structure of translation initiation factor 5B from the crenarchaeon Aeropyrum pernix. Proteins. 2016 May;84(5):712-7. doi: 10.1002/prot.25009. Epub 2016 Mar 6. PMID:26868175 doi:http://dx.doi.org/10.1002/prot.25009
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