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5j4g

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Crystal structure of the C-terminally His6-tagged HP0902, an uncharacterized protein from Helicobacter pylori 26695

Structural highlights

5j4g is a 2 chain structure with sequence from Helicobacter pylori 26695. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O25562_HELPY

Publication Abstract from PubMed

We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-beta cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry and NMR titration demonstrated that HP0902 is able to bind bacterial endotoxin lipopolysaccharides (LPS) through its surface-exposed loops, where metal-binding sites are usually found in other metallo-cupins. This report constitutes the first identification of an LPS-interacting protein, both in the cupin family and in H. pylori. Furthermore, identification of the ability of HP0902 to bind LPS uncovers a putative role for this protein in H. pylori pathogenicity.

Structural identification of the lipopolysaccharide-binding capability of a cupin-family protein from Helicobacter pylori.,Sim DW, Kim JH, Kim HY, Jang JH, Lee WC, Kim EH, Park PJ, Lee KH, Won HS FEBS Lett. 2016 Sep;590(17):2997-3004. doi: 10.1002/1873-3468.12332. Epub 2016, Aug 11. PMID:27466800^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sim DW, Kim JH, Kim HY, Jang JH, Lee WC, Kim EH, Park PJ, Lee KH, Won HS. Structural identification of the lipopolysaccharide-binding capability of a cupin-family protein from Helicobacter pylori. FEBS Lett. 2016 Sep;590(17):2997-3004. doi: 10.1002/1873-3468.12332. Epub 2016, Aug 11. PMID:27466800 doi:http://dx.doi.org/10.1002/1873-3468.12332

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5j4g"

@@ Line 3: / Line 3: @@
 <StructureSection load='5j4g' size='340' side='right'caption='[[5j4g]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5j4g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori Campylobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J4G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5j4g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J4G FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5j4f|5j4f]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HP_0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85962 Campylobacter pylori])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4g OCA], [https://pdbe.org/5j4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j4g RCSB], [https://www.ebi.ac.uk/pdbsum/5j4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4g ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4g OCA], [http://pdbe.org/5j4g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4g RCSB], [http://www.ebi.ac.uk/pdbsum/5j4g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4g ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O25562_HELPY O25562_HELPY]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Campylobacter pylori]]
+[[Category: Helicobacter pylori 26695]]
 [[Category: Large Structures]]
-[[Category: Kim, H Y]]
+[[Category: Kim HY]]
-[[Category: Kim, J H]]
+[[Category: Kim JH]]
-[[Category: Lee, W C]]
+[[Category: Lee WC]]
-[[Category: Sim, D W]]
+[[Category: Sim DW]]
-[[Category: Won, H S]]
+[[Category: Won HS]]
-[[Category: Cupin family]]
-[[Category: Helicobacter pylori]]
-[[Category: Hp0902]]
-[[Category: Secretory protein]]
-[[Category: Uncharacterized protein]]
-[[Category: Unknown function]]

5j4g

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Current revision

Crystal structure of the C-terminally His6-tagged HP0902, an uncharacterized protein from Helicobacter pylori 26695

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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