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6o14

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Crystal structure of the Aquifex aeolicus Wzt Carbohydrate Binding Domain

Structural highlights

6o14 is a 2 chain structure with sequence from Aquifex aeolicus VF5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.65Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O67181_AQUAE

Publication Abstract from PubMed

A hallmark of Gram-negative bacteria is an asymmetric outer membrane containing lipopolysaccharides (LPSs) in the extracellular leaflet. LPS molecules consist of lipid A, which is connected to the inner and outer core oligosaccharides. This LPS core structure is extended in the periplasm by the O antigen, a variable and serotype-defining polysaccharide. In the ABC transporter-dependent LPS biosynthesis pathway, the WzmWzt transporter secretes the complete O antigen across the inner membrane for ligation to the LPS core. In some O antigen transporters, the nucleotide-binding domain of Wzt is fused C-terminally to a carbohydrate-binding domain (CBD) that interacts with the O antigen chain. Here, we present the crystal structure of the Aquifex aeolicus CBD that reveals a conserved flat and a variable twisted jelly-roll surface. The CBD dimer is stabilized by mutual beta strand exchange. Microbial glycan array binding studies with the isolated CBD provide insights into its interaction with complex carbohydrates.

Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain.,Bi Y, Zimmer J Structure. 2020 Feb 4;28(2):252-258.e2. doi: 10.1016/j.str.2019.11.020. Epub 2019, Dec 23. PMID:31879128^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bi Y, Zimmer J. Structure and Ligand-Binding Properties of the O Antigen ABC Transporter Carbohydrate-Binding Domain. Structure. 2020 Feb 4;28(2):252-258.e2. doi: 10.1016/j.str.2019.11.020. Epub 2019, Dec 23. PMID:31879128 doi:http://dx.doi.org/10.1016/j.str.2019.11.020

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6o14"

Categories: Aquifex aeolicus VF5 | Large Structures | Bi Y | Zimmer J

@@ Line 3: / Line 3: @@
 <StructureSection load='6o14' size='340' side='right'caption='[[6o14]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6o14]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aquae Aquae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O14 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6O14 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6o14]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O14 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O14 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2r5o|2r5o]], [[5hno|5hno]], [[6an7|6an7]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">abcT4, aq_1094 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224324 AQUAE])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o14 OCA], [https://pdbe.org/6o14 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o14 RCSB], [https://www.ebi.ac.uk/pdbsum/6o14 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o14 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6o14 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o14 OCA], [http://pdbe.org/6o14 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6o14 RCSB], [http://www.ebi.ac.uk/pdbsum/6o14 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6o14 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O67181_AQUAE O67181_AQUAE]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Aquae]]
+[[Category: Aquifex aeolicus VF5]]
 [[Category: Large Structures]]
-[[Category: Bi, Y]]
+[[Category: Bi Y]]
-[[Category: Zimmer, J]]
+[[Category: Zimmer J]]
-[[Category: Abc transporter]]
-[[Category: Carbohydrate binding domain]]
-[[Category: Membrane protein]]
-[[Category: O antigen]]
-[[Category: Wzm]]
-[[Category: Wzt]]

6o14

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Current revision

Crystal structure of the Aquifex aeolicus Wzt Carbohydrate Binding Domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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