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2blx

From Proteopedia

(Difference between revisions)

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Revision as of 04:38, 19 November 2020

HEWL before a high dose x-ray "burn"

Structural highlights

2blx is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	132l, 193l, 194l, 1a2y, 1aki, 1at5, 1at6, 1azf, 1b0d, 1b2k, 1bgi, 1bhz, 1bvk, 1bvx, 1bwh, 1bwi, 1bwj, 1c08, 1c10, 1dpw, 1dpx, 1dqj, 1e8l, 1f0w, 1f10, 1f3j, 1fdl, 1flq, 1flu, 1flw, 1fly, 1fn5, 1g7h, 1g7i, 1g7j, 1g7l, 1g7m, 1gpq, 1gwd, 1gxv, 1gxx, 1h6m, 1h87, 1hc0, 1hel, 1hem, 1hen, 1heo, 1hep, 1heq, 1her, 1hew, 1hf4, 1hsw, 1hsx, 1ic4, 1ic5, 1ic7, 1iee, 1io5, 1ioq, 1ior, 1ios, 1iot, 1ir7, 1ir8, 1ir9, 1j1o, 1j1p, 1j1x, 1ja2, 1ja4, 1ja6, 1ja7, 1jis, 1jit, 1jiy, 1jj0, 1jj1, 1jj3, 1jpo, 1jto, 1jtt, 1kip, 1kiq, 1kir, 1kxw, 1kxx, 1kxy, 1lcn, 1lj3, 1lj4, 1lje, 1ljf, 1ljg, 1ljh, 1lji, 1ljj, 1ljk, 1lkr, 1lks, 1lma, 1lpi, 1lsa, 1lsb, 1lsc, 1lsd, 1lse, 1lsf, 1lsg, 1lsm, 1lsn, 1lsy, 1lsz, 1lyo, 1lys, 1lyz, 1lz8, 1lz9, 1lza, 1lzb, 1lzc, 1lzd, 1lze, 1lzg, 1lzh, 1lzn, 1lzt, 1mel, 1mlc, 1n4f, 1nby, 1nbz, 1ndg, 1ndm, 1p2c, 1ps5, 1qio, 1qtk, 1rcm, 1rfp, 1ri8, 1rjc, 1sf4, 1sf6, 1sf7, 1sfb, 1sfg, 1sq2, 1t6v, 1ua6, 1uc0, 1uco, 1uia, 1uib, 1uic, 1uid, 1uie, 1uif, 1uig, 1uih, 1uuz, 1v7s, 1v7t, 1vat, 1vau, 1vdp, 1vdq, 1vds, 1vdt, 1ved, 1vfb, 1w6z, 1wtm, 1wtn, 1xei, 1xej, 1xek, 1xfp, 1yik, 1yil, 2bly, 2cds, 2hfm, 2iff, 2lym, 2lyo, 2lyz, 2lzh, 2lzt, 3hfl, 3hfm, 3lym, 3lyo, 3lyt, 3lyz, 3lzt, 4lym, 4lyo, 4lyt, 4lyz, 4lzt, 5lym, 5lyt, 5lyz, 6lyt, 6lyz, 7lyz, 8lyz
Activity:	Lysozyme, with EC number 3.2.1.17
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Specific radiation damage can be used to solve macromolecular structures using the radiation-damage-induced phasing (RIP) method. The method has been investigated for six disulfide-containing test structures (elastase, insulin, lysozyme, ribonuclease A, trypsin and thaumatin) using data sets that were collected on a third-generation synchrotron undulator beamline with a highly attenuated beam. Each crystal was exposed to the unattenuated X-ray beam between the collection of a 'before' and an 'after' data set. The X-ray 'burn'-induced intensity differences ranged from 5 to 15%, depending on the protein investigated. X-ray-susceptible substructures were determined using the integrated direct and Patterson methods in SHELXD. The best substructures were found by downscaling the 'after' data set in SHELXC by a scale factor K, with optimal values ranging from 0.96 to 0.99. The initial substructures were improved through iteration with SHELXE by the addition of negatively occupied sites as well as a large number of relatively weak sites. The final substructures ranged from 40 to more than 300 sites, with strongest peaks as high as 57sigma. All structures except one could be solved: it was not possible to find the initial substructure for ribonuclease A, however, SHELXE iteration starting with the known five most susceptible sites gave excellent maps. Downscaling proved to be necessary for the solution of elastase, lysozyme and thaumatin and reduced the number of SHELXE iterations in the other cases. The combination of downscaling and substructure iteration provides important benefits for the phasing of macromolecular structures using radiation damage.

Improving radiation-damage substructures for RIP.,Nanao MH, Sheldrick GM, Ravelli RB Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1227-37. Epub 2005, Aug 16. PMID:16131756^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Nanao MH, Sheldrick GM, Ravelli RB. Improving radiation-damage substructures for RIP. Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1227-37. Epub 2005, Aug 16. PMID:16131756 doi:10.1107/S0907444905019360

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2blx"

@@ Line 3: / Line 3: @@
 <StructureSection load='2blx' size='340' side='right'caption='[[2blx]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2blx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BLX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2blx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2BLX FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[132l|132l]], [[193l|193l]], [[194l|194l]], [[1a2y|1a2y]], [[1aki|1aki]], [[1at5|1at5]], [[1at6|1at6]], [[1azf|1azf]], [[1b0d|1b0d]], [[1b2k|1b2k]], [[1bgi|1bgi]], [[1bhz|1bhz]], [[1bvk|1bvk]], [[1bvx|1bvx]], [[1bwh|1bwh]], [[1bwi|1bwi]], [[1bwj|1bwj]], [[1c08|1c08]], [[1c10|1c10]], [[1dpw|1dpw]], [[1dpx|1dpx]], [[1dqj|1dqj]], [[1e8l|1e8l]], [[1f0w|1f0w]], [[1f10|1f10]], [[1f3j|1f3j]], [[1fdl|1fdl]], [[1flq|1flq]], [[1flu|1flu]], [[1flw|1flw]], [[1fly|1fly]], [[1fn5|1fn5]], [[1g7h|1g7h]], [[1g7i|1g7i]], [[1g7j|1g7j]], [[1g7l|1g7l]], [[1g7m|1g7m]], [[1gpq|1gpq]], [[1gwd|1gwd]], [[1gxv|1gxv]], [[1gxx|1gxx]], [[1h6m|1h6m]], [[1h87|1h87]], [[1hc0|1hc0]], [[1hel|1hel]], [[1hem|1hem]], [[1hen|1hen]], [[1heo|1heo]], [[1hep|1hep]], [[1heq|1heq]], [[1her|1her]], [[1hew|1hew]], [[1hf4|1hf4]], [[1hsw|1hsw]], [[1hsx|1hsx]], [[1ic4|1ic4]], [[1ic5|1ic5]], [[1ic7|1ic7]], [[1iee|1iee]], [[1io5|1io5]], [[1ioq|1ioq]], [[1ior|1ior]], [[1ios|1ios]], [[1iot|1iot]], [[1ir7|1ir7]], [[1ir8|1ir8]], [[1ir9|1ir9]], [[1j1o|1j1o]], [[1j1p|1j1p]], [[1j1x|1j1x]], [[1ja2|1ja2]], [[1ja4|1ja4]], [[1ja6|1ja6]], [[1ja7|1ja7]], [[1jis|1jis]], [[1jit|1jit]], [[1jiy|1jiy]], [[1jj0|1jj0]], [[1jj1|1jj1]], [[1jj3|1jj3]], [[1jpo|1jpo]], [[1jto|1jto]], [[1jtt|1jtt]], [[1kip|1kip]], [[1kiq|1kiq]], [[1kir|1kir]], [[1kxw|1kxw]], [[1kxx|1kxx]], [[1kxy|1kxy]], [[1lcn|1lcn]], [[1lj3|1lj3]], [[1lj4|1lj4]], [[1lje|1lje]], [[1ljf|1ljf]], [[1ljg|1ljg]], [[1ljh|1ljh]], [[1lji|1lji]], [[1ljj|1ljj]], [[1ljk|1ljk]], [[1lkr|1lkr]], [[1lks|1lks]], [[1lma|1lma]], [[1lpi|1lpi]], [[1lsa|1lsa]], [[1lsb|1lsb]], [[1lsc|1lsc]], [[1lsd|1lsd]], [[1lse|1lse]], [[1lsf|1lsf]], [[1lsg|1lsg]], [[1lsm|1lsm]], [[1lsn|1lsn]], [[1lsy|1lsy]], [[1lsz|1lsz]], [[1lyo|1lyo]], [[1lys|1lys]], [[1lyz|1lyz]], [[1lz8|1lz8]], [[1lz9|1lz9]], [[1lza|1lza]], [[1lzb|1lzb]], [[1lzc|1lzc]], [[1lzd|1lzd]], [[1lze|1lze]], [[1lzg|1lzg]], [[1lzh|1lzh]], [[1lzn|1lzn]], [[1lzt|1lzt]], [[1mel|1mel]], [[1mlc|1mlc]], [[1n4f|1n4f]], [[1nby|1nby]], [[1nbz|1nbz]], [[1ndg|1ndg]], [[1ndm|1ndm]], [[1p2c|1p2c]], [[1ps5|1ps5]], [[1qio|1qio]], [[1qtk|1qtk]], [[1rcm|1rcm]], [[1rfp|1rfp]], [[1ri8|1ri8]], [[1rjc|1rjc]], [[1sf4|1sf4]], [[1sf6|1sf6]], [[1sf7|1sf7]], [[1sfb|1sfb]], [[1sfg|1sfg]], [[1sq2|1sq2]], [[1t6v|1t6v]], [[1ua6|1ua6]], [[1uc0|1uc0]], [[1uco|1uco]], [[1uia|1uia]], [[1uib|1uib]], [[1uic|1uic]], [[1uid|1uid]], [[1uie|1uie]], [[1uif|1uif]], [[1uig|1uig]], [[1uih|1uih]], [[1uuz|1uuz]], [[1v7s|1v7s]], [[1v7t|1v7t]], [[1vat|1vat]], [[1vau|1vau]], [[1vdp|1vdp]], [[1vdq|1vdq]], [[1vds|1vds]], [[1vdt|1vdt]], [[1ved|1ved]], [[1vfb|1vfb]], [[1w6z|1w6z]], [[1wtm|1wtm]], [[1wtn|1wtn]], [[1xei|1xei]], [[1xej|1xej]], [[1xek|1xek]], [[1xfp|1xfp]], [[1yik|1yik]], [[1yil|1yil]], [[2bly|2bly]], [[2cds|2cds]], [[2hfm|2hfm]], [[2iff|2iff]], [[2lym|2lym]], [[2lyo|2lyo]], [[2lyz|2lyz]], [[2lzh|2lzh]], [[2lzt|2lzt]], [[3hfl|3hfl]], [[3hfm|3hfm]], [[3lym|3lym]], [[3lyo|3lyo]], [[3lyt|3lyt]], [[3lyz|3lyz]], [[3lzt|3lzt]], [[4lym|4lym]], [[4lyo|4lyo]], [[4lyt|4lyt]], [[4lyz|4lyz]], [[4lzt|4lzt]], [[5lym|5lym]], [[5lyt|5lyt]], [[5lyz|5lyz]], [[6lyt|6lyt]], [[6lyz|6lyz]], [[7lyz|7lyz]], [[8lyz|8lyz]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[132l|132l]], [[193l|193l]], [[194l|194l]], [[1a2y|1a2y]], [[1aki|1aki]], [[1at5|1at5]], [[1at6|1at6]], [[1azf|1azf]], [[1b0d|1b0d]], [[1b2k|1b2k]], [[1bgi|1bgi]], [[1bhz|1bhz]], [[1bvk|1bvk]], [[1bvx|1bvx]], [[1bwh|1bwh]], [[1bwi|1bwi]], [[1bwj|1bwj]], [[1c08|1c08]], [[1c10|1c10]], [[1dpw|1dpw]], [[1dpx|1dpx]], [[1dqj|1dqj]], [[1e8l|1e8l]], [[1f0w|1f0w]], [[1f10|1f10]], [[1f3j|1f3j]], [[1fdl|1fdl]], [[1flq|1flq]], [[1flu|1flu]], [[1flw|1flw]], [[1fly|1fly]], [[1fn5|1fn5]], [[1g7h|1g7h]], [[1g7i|1g7i]], [[1g7j|1g7j]], [[1g7l|1g7l]], [[1g7m|1g7m]], [[1gpq|1gpq]], [[1gwd|1gwd]], [[1gxv|1gxv]], [[1gxx|1gxx]], [[1h6m|1h6m]], [[1h87|1h87]], [[1hc0|1hc0]], [[1hel|1hel]], [[1hem|1hem]], [[1hen|1hen]], [[1heo|1heo]], [[1hep|1hep]], [[1heq|1heq]], [[1her|1her]], [[1hew|1hew]], [[1hf4|1hf4]], [[1hsw|1hsw]], [[1hsx|1hsx]], [[1ic4|1ic4]], [[1ic5|1ic5]], [[1ic7|1ic7]], [[1iee|1iee]], [[1io5|1io5]], [[1ioq|1ioq]], [[1ior|1ior]], [[1ios|1ios]], [[1iot|1iot]], [[1ir7|1ir7]], [[1ir8|1ir8]], [[1ir9|1ir9]], [[1j1o|1j1o]], [[1j1p|1j1p]], [[1j1x|1j1x]], [[1ja2|1ja2]], [[1ja4|1ja4]], [[1ja6|1ja6]], [[1ja7|1ja7]], [[1jis|1jis]], [[1jit|1jit]], [[1jiy|1jiy]], [[1jj0|1jj0]], [[1jj1|1jj1]], [[1jj3|1jj3]], [[1jpo|1jpo]], [[1jto|1jto]], [[1jtt|1jtt]], [[1kip|1kip]], [[1kiq|1kiq]], [[1kir|1kir]], [[1kxw|1kxw]], [[1kxx|1kxx]], [[1kxy|1kxy]], [[1lcn|1lcn]], [[1lj3|1lj3]], [[1lj4|1lj4]], [[1lje|1lje]], [[1ljf|1ljf]], [[1ljg|1ljg]], [[1ljh|1ljh]], [[1lji|1lji]], [[1ljj|1ljj]], [[1ljk|1ljk]], [[1lkr|1lkr]], [[1lks|1lks]], [[1lma|1lma]], [[1lpi|1lpi]], [[1lsa|1lsa]], [[1lsb|1lsb]], [[1lsc|1lsc]], [[1lsd|1lsd]], [[1lse|1lse]], [[1lsf|1lsf]], [[1lsg|1lsg]], [[1lsm|1lsm]], [[1lsn|1lsn]], [[1lsy|1lsy]], [[1lsz|1lsz]], [[1lyo|1lyo]], [[1lys|1lys]], [[1lyz|1lyz]], [[1lz8|1lz8]], [[1lz9|1lz9]], [[1lza|1lza]], [[1lzb|1lzb]], [[1lzc|1lzc]], [[1lzd|1lzd]], [[1lze|1lze]], [[1lzg|1lzg]], [[1lzh|1lzh]], [[1lzn|1lzn]], [[1lzt|1lzt]], [[1mel|1mel]], [[1mlc|1mlc]], [[1n4f|1n4f]], [[1nby|1nby]], [[1nbz|1nbz]], [[1ndg|1ndg]], [[1ndm|1ndm]], [[1p2c|1p2c]], [[1ps5|1ps5]], [[1qio|1qio]], [[1qtk|1qtk]], [[1rcm|1rcm]], [[1rfp|1rfp]], [[1ri8|1ri8]], [[1rjc|1rjc]], [[1sf4|1sf4]], [[1sf6|1sf6]], [[1sf7|1sf7]], [[1sfb|1sfb]], [[1sfg|1sfg]], [[1sq2|1sq2]], [[1t6v|1t6v]], [[1ua6|1ua6]], [[1uc0|1uc0]], [[1uco|1uco]], [[1uia|1uia]], [[1uib|1uib]], [[1uic|1uic]], [[1uid|1uid]], [[1uie|1uie]], [[1uif|1uif]], [[1uig|1uig]], [[1uih|1uih]], [[1uuz|1uuz]], [[1v7s|1v7s]], [[1v7t|1v7t]], [[1vat|1vat]], [[1vau|1vau]], [[1vdp|1vdp]], [[1vdq|1vdq]], [[1vds|1vds]], [[1vdt|1vdt]], [[1ved|1ved]], [[1vfb|1vfb]], [[1w6z|1w6z]], [[1wtm|1wtm]], [[1wtn|1wtn]], [[1xei|1xei]], [[1xej|1xej]], [[1xek|1xek]], [[1xfp|1xfp]], [[1yik|1yik]], [[1yil|1yil]], [[2bly|2bly]], [[2cds|2cds]], [[2hfm|2hfm]], [[2iff|2iff]], [[2lym|2lym]], [[2lyo|2lyo]], [[2lyz|2lyz]], [[2lzh|2lzh]], [[2lzt|2lzt]], [[3hfl|3hfl]], [[3hfm|3hfm]], [[3lym|3lym]], [[3lyo|3lyo]], [[3lyt|3lyt]], [[3lyz|3lyz]], [[3lzt|3lzt]], [[4lym|4lym]], [[4lyo|4lyo]], [[4lyt|4lyt]], [[4lyz|4lyz]], [[4lzt|4lzt]], [[5lym|5lym]], [[5lyt|5lyt]], [[5lyz|5lyz]], [[6lyt|6lyt]], [[6lyz|6lyz]], [[7lyz|7lyz]], [[8lyz|8lyz]]</div></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2blx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blx OCA], [http://pdbe.org/2blx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2blx RCSB], [http://www.ebi.ac.uk/pdbsum/2blx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2blx ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2blx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blx OCA], [http://pdbe.org/2blx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2blx RCSB], [http://www.ebi.ac.uk/pdbsum/2blx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2blx ProSAT]</span></td></tr>
 </table>
 == Function ==

2blx

From Proteopedia

Revision as of 04:38, 19 November 2020

HEWL before a high dose x-ray "burn"

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

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