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| | <StructureSection load='5b0u' size='340' side='right'caption='[[5b0u]], [[Resolution|resolution]] 1.71Å' scene=''> | | <StructureSection load='5b0u' size='340' side='right'caption='[[5b0u]], [[Resolution|resolution]] 1.71Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5b0u]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Luminous_shrimp Luminous shrimp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B0U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5b0u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oplophorus_gracilirostris Oplophorus gracilirostris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B0U FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0u OCA], [http://pdbe.org/5b0u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b0u RCSB], [http://www.ebi.ac.uk/pdbsum/5b0u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0u ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b0u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0u OCA], [https://pdbe.org/5b0u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b0u RCSB], [https://www.ebi.ac.uk/pdbsum/5b0u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0u ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LUCI_OPLGR LUCI_OPLGR]] Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine.<ref>PMID:10984608</ref> | + | [https://www.uniprot.org/uniprot/LUCI_OPLGR LUCI_OPLGR] Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine.<ref>PMID:10984608</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Luminous shrimp]] | + | [[Category: Oplophorus gracilirostris]] |
| - | [[Category: Ehara, H]] | + | [[Category: Ehara H]] |
| - | [[Category: Sekine, S I]] | + | [[Category: Sekine SI]] |
| - | [[Category: Shirouzu, M]] | + | [[Category: Shirouzu M]] |
| - | [[Category: Tomabechi, Y]] | + | [[Category: Tomabechi Y]] |
| - | [[Category: Luminescent protein]]
| + | |
| - | [[Category: Oplophorus luciferase]]
| + | |
| Structural highlights
Function
LUCI_OPLGR Catalytic subunit of oplophorus-luciferin 2-monooxygenase. Oxidoreductase that converts coelenterazine (the oplophorus luciferin) to coelenteramide under emission of blue light with a maximum at 454 nm. Is also active with bisdeoxycoelenterazine.[1]
Publication Abstract from PubMed
The 19 kDa protein (KAZ) of Oplophorus luciferase is a catalytic component, that oxidizes coelenterazine (a luciferin) with molecular oxygen to emit light. The crystal structure of the mutated 19 kDa protein (nanoKAZ) was determined at 1.71 A resolution. The structure consists of 11 antiparallel beta-strands forming a beta-barrel that is capped by 4 short alpha-helices. The structure of nanoKAZ is similar to those of fatty acid-binding proteins (FABPs), even though the amino acid sequence similarity was very low between them. The coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the beta-barrel structure.
Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine.,Tomabechi Y, Hosoya T, Ehara H, Sekine S, Shirouzu M, Inouye S Biochem Biophys Res Commun. 2016 Jan 29;470(1):88-93. doi:, 10.1016/j.bbrc.2015.12.123. Epub 2015 Dec 30. PMID:26746005[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Inouye S, Watanabe K, Nakamura H, Shimomura O. Secretional luciferase of the luminous shrimp Oplophorus gracilirostris: cDNA cloning of a novel imidazopyrazinone luciferase(1). FEBS Lett. 2000 Sep 8;481(1):19-25. PMID:10984608
- ↑ Tomabechi Y, Hosoya T, Ehara H, Sekine S, Shirouzu M, Inouye S. Crystal structure of nanoKAZ: The mutated 19 kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine. Biochem Biophys Res Commun. 2016 Jan 29;470(1):88-93. doi:, 10.1016/j.bbrc.2015.12.123. Epub 2015 Dec 30. PMID:26746005 doi:http://dx.doi.org/10.1016/j.bbrc.2015.12.123
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