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| | <StructureSection load='5b86' size='340' side='right'caption='[[5b86]], [[Resolution|resolution]] 3.02Å' scene=''> | | <StructureSection load='5b86' size='340' side='right'caption='[[5b86]], [[Resolution|resolution]] 3.02Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5b86]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B86 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B86 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5b86]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B86 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tnfaip2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b86 OCA], [https://pdbe.org/5b86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b86 RCSB], [https://www.ebi.ac.uk/pdbsum/5b86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b86 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b86 OCA], [http://pdbe.org/5b86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b86 RCSB], [http://www.ebi.ac.uk/pdbsum/5b86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b86 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TNAP2_MOUSE TNAP2_MOUSE]] May play a role as a mediator of inflammation and angiogenesis. | + | [https://www.uniprot.org/uniprot/TNAP2_MOUSE TNAP2_MOUSE] May play a role as a mediator of inflammation and angiogenesis. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Fukai, S]] | + | [[Category: Fukai S]] |
| - | [[Category: Sato, Y]] | + | [[Category: Sato Y]] |
| - | [[Category: Yamagata, A]] | + | [[Category: Yamagata A]] |
| - | [[Category: Yamashita, M]] | + | [[Category: Yamashita M]] |
| - | [[Category: Exocyst complex]]
| + | |
| - | [[Category: Exocytosis]]
| + | |
| - | [[Category: Helical protein]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Membrane traffic]]
| + | |
| Structural highlights
Function
TNAP2_MOUSE May play a role as a mediator of inflammation and angiogenesis.
Publication Abstract from PubMed
The tunneling nanotube (TNT) is a structure used for intercellular communication, and is a thin membrane protrusion mediating transport of various signaling molecules and cellular components. M-Sec has potent membrane deformation ability and induces TNT formation in cooperation with the Ral/exocyst complex. Here, we show that the N-terminal polybasic region of M-Sec directly binds phosphatidylinositol (4,5)-bisphosphate for its localization to the plasma membrane during the initial stage of TNT formation. We further report a crystal structure of M-Sec, which consists of helix bundles arranged in a straight rod-like shape, similar to the membrane tethering complex subunits. A positively charged surface in the C-terminal domains is required for M-Sec interaction with active RalA to extend the plasma membrane protrusions. Our results suggest that the membrane-associated M-Sec recruits active RalA, which directs the exocyst complex to form TNTs.
Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation.,Kimura S, Yamashita M, Yamakami-Kimura M, Sato Y, Yamagata A, Kobashigawa Y, Inagaki F, Amada T, Hase K, Iwanaga T, Ohno H, Fukai S Sci Rep. 2016 Sep 15;6:33548. doi: 10.1038/srep33548. PMID:27629377[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kimura S, Yamashita M, Yamakami-Kimura M, Sato Y, Yamagata A, Kobashigawa Y, Inagaki F, Amada T, Hase K, Iwanaga T, Ohno H, Fukai S. Distinct Roles for the N- and C-terminal Regions of M-Sec in Plasma Membrane Deformation during Tunneling Nanotube Formation. Sci Rep. 2016 Sep 15;6:33548. doi: 10.1038/srep33548. PMID:27629377 doi:http://dx.doi.org/10.1038/srep33548
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