|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5h69' size='340' side='right'caption='[[5h69]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='5h69' size='340' side='right'caption='[[5h69]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5h69]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_stearothermophilus_10 Bacillus stearothermophilus 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5H69 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5h69]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus_10 Geobacillus stearothermophilus 10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H69 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h66|5h66]], [[5h67|5h67]], [[5h68|5h68]], [[3w6j|3w6j]], [[3w6k|3w6k]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2004Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">smc, GT50_02560 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272567 Bacillus stearothermophilus 10])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h69 OCA], [https://pdbe.org/5h69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h69 RCSB], [https://www.ebi.ac.uk/pdbsum/5h69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h69 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h69 OCA], [http://pdbe.org/5h69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h69 RCSB], [http://www.ebi.ac.uk/pdbsum/5h69 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h69 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | == Function == | |
| - | [[http://www.uniprot.org/uniprot/A0A0K2H586_GEOSE A0A0K2H586_GEOSE]] Required for chromosome condensation and partitioning.[HAMAP-Rule:MF_01894] | |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 20: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus stearothermophilus 10]] | + | [[Category: Geobacillus stearothermophilus 10]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hirano, T]] | + | [[Category: Hirano T]] |
| - | [[Category: Kamada, K]] | + | [[Category: Kamada K]] |
| - | [[Category: Cell cycle]]
| + | |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Smc protein]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The SMC-ScpAB complex plays a crucial role in chromosome organization and segregation in many bacteria. It is composed of a V-shaped SMC dimer and an ScpAB subcomplex that bridges the two Structural Maintenance of Chromosomes (SMC) head domains. Despite its functional significance, the mechanistic details of SMC-ScpAB remain obscure. Here we provide evidence that ATP-dependent head-head engagement induces a lever movement of the SMC neck region, which might help to separate juxtaposed coiled-coil arms. Binding of the ScpA N-terminal domain (NTD) to the SMC neck region is negatively regulated by the ScpB C-terminal domain. Mutations in the ScpA NTD compromise this regulation and profoundly affect the overall shape of the complex. The SMC hinge domain is structurally relaxed when free from coiled-coil juxtaposition. Taken together, we propose that the structural parts of SMC-ScpAB are subjected to the balance between constraint and relaxation, cooperating to modulate dynamic conformational changes of the whole complex.
Overall Shapes of the SMC-ScpAB Complex Are Determined by Balance between Constraint and Relaxation of Its Structural Parts.,Kamada K, Su'etsugu M, Takada H, Miyata M, Hirano T Structure. 2017 Apr 4;25(4):603-616.e4. doi: 10.1016/j.str.2017.02.008. Epub 2017, Mar 9. PMID:28286005[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kamada K, Su'etsugu M, Takada H, Miyata M, Hirano T. Overall Shapes of the SMC-ScpAB Complex Are Determined by Balance between Constraint and Relaxation of Its Structural Parts. Structure. 2017 Apr 4;25(4):603-616.e4. doi: 10.1016/j.str.2017.02.008. Epub 2017, Mar 9. PMID:28286005 doi:http://dx.doi.org/10.1016/j.str.2017.02.008
|
