6ihj

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of Drosophila Nxf1 NTF2 domain in complex with Nxt1/p15

Structural highlights

6ihj is a 4 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NXT1_DROME Stimulator of protein export for NES-containing proteins (By similarity). Plays a role in the nuclear export of mRNA (PubMed:14729961). Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA (By similarity).[UniProtKB:Q9UKK6]^[1]

Publication Abstract from PubMed

The repression of transposons by the Piwi-interacting RNA (piRNA) pathway is essential to protect animal germ cells. In Drosophila, Panoramix enforces transcriptional silencing by binding to the target-engaged Piwi-piRNA complex, although the precise mechanisms by which this occurs remain elusive. Here, we show that Panoramix functions together with a germline-specific paralogue of a nuclear export factor, dNxf2, and its cofactor dNxt1 (p15), to suppress transposon expression. The transposon RNA-binding protein dNxf2 is required for animal fertility and Panoramix-mediated silencing. Transient tethering of dNxf2 to nascent transcripts leads to their nuclear retention. The NTF2 domain of dNxf2 competes dNxf1 (TAP) off nucleoporins, a process required for proper RNA export. Thus, dNxf2 functions in a Panoramix-dNxf2-dependent TAP/p15 silencing (Pandas) complex that counteracts the canonical RNA exporting machinery and restricts transposons to the nuclear peripheries. Our findings may have broader implications for understanding how RNA metabolism modulates heterochromatin formation.

A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation.,Zhao K, Cheng S, Miao N, Xu P, Lu X, Zhang Y, Wang M, Ouyang X, Yuan X, Liu W, Lu X, Zhou P, Gu J, Zhang Y, Qiu D, Jin Z, Su C, Peng C, Wang JH, Dong MQ, Wan Y, Ma J, Cheng H, Huang Y, Yu Y Nat Cell Biol. 2019 Oct;21(10):1261-1272. doi: 10.1038/s41556-019-0396-0. Epub, 2019 Sep 30. PMID:31570835^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Forler D, Rabut G, Ciccarelli FD, Herold A, Kocher T, Niggeweg R, Bork P, Ellenberg J, Izaurralde E. RanBP2/Nup358 provides a major binding site for NXF1-p15 dimers at the nuclear pore complex and functions in nuclear mRNA export. Mol Cell Biol. 2004 Feb;24(3):1155-67. doi: 10.1128/mcb.24.3.1155-1167.2004. PMID:14729961 doi:http://dx.doi.org/10.1128/mcb.24.3.1155-1167.2004
↑ Zhao K, Cheng S, Miao N, Xu P, Lu X, Zhang Y, Wang M, Ouyang X, Yuan X, Liu W, Lu X, Zhou P, Gu J, Zhang Y, Qiu D, Jin Z, Su C, Peng C, Wang JH, Dong MQ, Wan Y, Ma J, Cheng H, Huang Y, Yu Y. A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation. Nat Cell Biol. 2019 Oct;21(10):1261-1272. doi: 10.1038/s41556-019-0396-0. Epub, 2019 Sep 30. PMID:31570835 doi:http://dx.doi.org/10.1038/s41556-019-0396-0

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ihj"

Categories: Drosophila melanogaster | Large Structures | Huang Y | Yuan X

@@ Line 3: / Line 3: @@
 <StructureSection load='6ihj' size='340' side='right'caption='[[6ihj]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ihj]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IHJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ihj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IHJ FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sbr, nxf1, CG1664 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME]), Nxt1, CG12752 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ihj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ihj OCA], [http://pdbe.org/6ihj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ihj RCSB], [http://www.ebi.ac.uk/pdbsum/6ihj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ihj ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ihj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ihj OCA], [https://pdbe.org/6ihj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ihj RCSB], [https://www.ebi.ac.uk/pdbsum/6ihj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ihj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/NXF1_DROME NXF1_DROME]] Mediates the export of the majority of mRNAs from the nucleus to the cytoplasm.<ref>PMID:11779805</ref> <ref>PMID:11780633</ref> <ref>PMID:11780634</ref>  [[http://www.uniprot.org/uniprot/NXT1_DROME NXT1_DROME]] Stimulator of protein export for NES-containing proteins (By similarity). Plays a role in the nuclear export of mRNA (PubMed:14729961). Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA (By similarity).[UniProtKB:Q9UKK6]<ref>PMID:14729961</ref>
+[https://www.uniprot.org/uniprot/NXT1_DROME NXT1_DROME] Stimulator of protein export for NES-containing proteins (By similarity). Plays a role in the nuclear export of mRNA (PubMed:14729961). Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA (By similarity).[UniProtKB:Q9UKK6]<ref>PMID:14729961</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
 [[Category: Large Structures]]
-[[Category: Huang, Y]]
+[[Category: Huang Y]]
-[[Category: Yuan, X]]
+[[Category: Yuan X]]
-[[Category: Drosophila]]
-[[Category: Ntf2 domain]]
-[[Category: Nuclear export factor]]
-[[Category: Nxf2]]
-[[Category: Nxt1]]
-[[Category: P15]]
-[[Category: Protein transport]]

6ihj

From Proteopedia

Current revision

Crystal structure of Drosophila Nxf1 NTF2 domain in complex with Nxt1/p15

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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