1tge

From Proteopedia

(Difference between revisions)

Revision as of 16:40, 10 April 2020

The structure of immature Dengue virus at 12.5 angstrom

1tge, resolution 12.50Å

Structural highlights

1tge is a 3 chain structure with sequence from Dengue virus 2 puerto rico/pr159-s1/1969. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1tg8, 1thd
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[POLG_DEN2T] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity). Non-structural protein 1 is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome (By similarity). Non-structural protein 2A may be involved viral RNA replication and capsid assembly (Potential). Non-structural protein 2B is a required cofactor for the serine protease function of NS3 (By similarity). Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity).

Publication Abstract from PubMed

Dengue virus, a member of the Flaviviridae family, has a surface composed of 180 copies each of the envelope (E) glycoprotein and the membrane (M) protein. The crystal structure of an N-terminal fragment of E has been determined and compared with a previously described structure. The primary difference between these structures is a 10 degrees rotation about a hinge relating the fusion domain DII to domains DI and DIII. These two rigid body components were used for independent fitting of E into the cryo-electron microscopy maps of both immature and mature dengue viruses. The fitted E structures in these two particles showed a difference of 27 degrees between the two components. Comparison of the E structure in its postfusion state with that in the immature and mature virions shows a rotation approximately around the same hinge. Flexibility of E is apparently a functional requirement for assembly and infection of flaviviruses.

Conformational changes of the flavivirus E glycoprotein.,Zhang Y, Zhang W, Ogata S, Clements D, Strauss JH, Baker TS, Kuhn RJ, Rossmann MG Structure. 2004 Sep;12(9):1607-18. PMID:15341726^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang Y, Zhang W, Ogata S, Clements D, Strauss JH, Baker TS, Kuhn RJ, Rossmann MG. Conformational changes of the flavivirus E glycoprotein. Structure. 2004 Sep;12(9):1607-18. PMID:15341726 doi:10.1016/j.str.2004.06.019

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tge"

@@ Line 3: / Line 3: @@
 <SX load='1tge' size='340' side='right' viewer='molstar' caption='[[1tge]], [[Resolution|resolution]] 12.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1tge]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2_puerto_rico/pr159-s1/1969 Dengue virus 2 puerto rico/pr159-s1/1969]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TGE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1tge]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_2_puerto_rico/pr159-s1/1969 Dengue virus 2 puerto rico/pr159-s1/1969]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1TGE FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1tg8|1tg8]], [[1thd|1thd]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tge OCA], [http://pdbe.org/1tge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tge RCSB], [http://www.ebi.ac.uk/pdbsum/1tge PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tge ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1tge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tge OCA], [http://pdbe.org/1tge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tge RCSB], [http://www.ebi.ac.uk/pdbsum/1tge PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tge ProSAT]</span></td></tr>
 </table>
 == Function ==

1tge

From Proteopedia

Revision as of 16:40, 10 April 2020

The structure of immature Dengue virus at 12.5 angstrom

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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