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1trj

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Revision as of 16:41, 10 April 2020

Homology Model of Yeast RACK1 Protein fitted into 11.7A cryo-EM map of Yeast 80S Ribosome

1trj, resolution 11.70Å

Structural highlights

1trj is a 3 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1got, 1s1h
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GBLP_YEAST] Located at the head of the 40S ribosomal subunit in the vicinity of the mRNA exit channel, it serves as a scaffold protein that can recruit other proteins to the ribosome. Involved in the negative regulation of translation of a specific subset of proteins.^[1]

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

RACK1 serves as a scaffold protein for a wide range of kinases and membrane-bound receptors. It is a WD-repeat family protein and is predicted to have a beta-propeller architecture with seven blades like a Gbeta protein. Mass spectrometry studies have identified its association with the small subunit of eukaryotic ribosomes and, most recently, it has been shown to regulate initiation by recruiting protein kinase C to the 40S subunit. Here we present the results of a cryo-EM study of the 80S ribosome that positively locate RACK1 on the head region of the 40S subunit, in the immediate vicinity of the mRNA exit channel. One face of RACK1 exposes the WD-repeats as a platform for interactions with kinases and receptors. Using this platform, RACK1 can recruit other proteins to the ribosome.

Identification of the versatile scaffold protein RACK1 on the eukaryotic ribosome by cryo-EM.,Sengupta J, Nilsson J, Gursky R, Spahn CM, Nissen P, Frank J Nat Struct Mol Biol. 2004 Oct;11(10):957-62. Epub 2004 Aug 29. PMID:15334071^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gerbasi VR, Weaver CM, Hill S, Friedman DB, Link AJ. Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Mol Cell Biol. 2004 Sep;24(18):8276-87. PMID:15340087 doi:10.1128/MCB.24.18.8276-8287.2004
↑ Sengupta J, Nilsson J, Gursky R, Spahn CM, Nissen P, Frank J. Identification of the versatile scaffold protein RACK1 on the eukaryotic ribosome by cryo-EM. Nat Struct Mol Biol. 2004 Oct;11(10):957-62. Epub 2004 Aug 29. PMID:15334071 doi:10.1038/nsmb822

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1trj"

@@ Line 3: / Line 3: @@
 <SX load='1trj' size='340' side='right' viewer='molstar' caption='[[1trj]], [[Resolution|resolution]] 11.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1trj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TRJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TRJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1trj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TRJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1TRJ FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1got|1got]], [[1s1h|1s1h]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1trj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1trj OCA], [http://pdbe.org/1trj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1trj RCSB], [http://www.ebi.ac.uk/pdbsum/1trj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1trj ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1trj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1trj OCA], [http://pdbe.org/1trj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1trj RCSB], [http://www.ebi.ac.uk/pdbsum/1trj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1trj ProSAT]</span></td></tr>
 </table>
 == Function ==

1trj

From Proteopedia

Revision as of 16:41, 10 April 2020

Homology Model of Yeast RACK1 Protein fitted into 11.7A cryo-EM map of Yeast 80S Ribosome

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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