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3j22

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Revision as of 17:44, 10 April 2020

The Enterovirus 71 A-particle

3j22, resolution 6.30Å

Structural highlights

3j22 is a 3 chain structure with sequence from Human enterovirus 71. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3j23
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[B2ZUN0_9ENTO] Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).[SAAS:SAAS000199_004_016611] Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease (By similarity).[SAAS:SAAS000199_004_042266] RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals (By similarity).[SAAS:SAAS000199_004_010047]

Publication Abstract from PubMed

Since its discovery in 1969, enterovirus 71 (EV71) has emerged as a serious worldwide health threat. This human pathogen of the picornavirus family causes hand, foot, and mouth disease, and also has the capacity to invade the central nervous system to cause severe disease and death. Upon binding to a host receptor on the cell surface, the virus begins a two-step uncoating process, first forming an expanded, altered "A-particle", which is primed for genome release. In a second step after endocytosis, an unknown trigger leads to RNA expulsion, generating an intact, empty capsid. Cryo-electron microscopy reconstructions of these two capsid states provide insight into the mechanics of genome release. The EV71 A-particle capsid interacts with the genome near the icosahedral two-fold axis of symmetry, which opens to the external environment via a channel approximately 10 A in diameter that is lined with patches of negatively charged residues. After the EV71 genome has been released, the two-fold channel shrinks, though the overall capsid dimensions are conserved. These structural characteristics identify the two-fold channel as the site where a gateway forms and regulates the process of genome release.

The Enterovirus 71 A-particle Forms a Gateway to Allow Genome Release: A CryoEM Study of Picornavirus Uncoating.,Shingler KL, Yoder JL, Carnegie MS, Ashley RE, Makhov AM, Conway JF, Hafenstein S PLoS Pathog. 2013 Mar;9(3):e1003240. doi: 10.1371/journal.ppat.1003240. Epub 2013, Mar 21. PMID:23555253^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shingler KL, Yoder JL, Carnegie MS, Ashley RE, Makhov AM, Conway JF, Hafenstein S. The Enterovirus 71 A-particle Forms a Gateway to Allow Genome Release: A CryoEM Study of Picornavirus Uncoating. PLoS Pathog. 2013 Mar;9(3):e1003240. doi: 10.1371/journal.ppat.1003240. Epub 2013, Mar 21. PMID:23555253 doi:http://dx.doi.org/10.1371/journal.ppat.1003240

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j22"

Categories: Human enterovirus 71 | Large Structures | Shingler, K L | Ev71 | Virus

@@ Line 3: / Line 3: @@
 <SX load='3j22' size='340' side='right' viewer='molstar' caption='[[3j22]], [[Resolution|resolution]] 6.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3j22]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_enterovirus_71 Human enterovirus 71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J22 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J22 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3j22]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_enterovirus_71 Human enterovirus 71]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J22 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J22 FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j23|3j23]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j22 OCA], [http://pdbe.org/3j22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j22 RCSB], [http://www.ebi.ac.uk/pdbsum/3j22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j22 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j22 OCA], [http://pdbe.org/3j22 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j22 RCSB], [http://www.ebi.ac.uk/pdbsum/3j22 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j22 ProSAT]</span></td></tr>
 </table>
 == Function ==

3j22

From Proteopedia

Revision as of 17:44, 10 April 2020

The Enterovirus 71 A-particle

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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