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3j2u

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Revision as of 17:49, 10 April 2020

Kinesin-13 KLP10A HD in complex with CS-tubulin and a microtubule

3j2u, resolution 10.80Å

Structural highlights

3j2u is a 5 chain structure with sequence from Bos taurus and Drome. The July 2014 RCSB PDB Molecule of the Month feature on Microtubules by David Goodsell is 10.2210/rcsb_pdb/mom_2014_7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	Klp10A, CG1453 (DROME)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KI10A_DROME] Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux".^[1] [TBB2B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Publication Abstract from PubMed

To elucidate the structural basis of the mechanism of microtubule depolymerization by kinesin-13s, we analyzed complexes of tubulin and the Drosophila melanogaster kinesin-13 KLP10A by electron microscopy (EM) and fluorescence polarization microscopy. We report a nanometer-resolution (1.1 nm) cryo-EM three-dimensional structure of the KLP10A head domain (KLP10AHD) bound to curved tubulin. We found that binding of KLP10AHD induces a distinct tubulin configuration with displacement (shear) between tubulin subunits in addition to curvature. In this configuration, the kinesin-binding site differs from that in straight tubulin, providing an explanation for the distinct interaction modes of kinesin-13s with the microtubule lattice or its ends. The KLP10AHD-tubulin interface comprises three areas of interaction, suggesting a crossbow-type tubulin-bending mechanism. These areas include the kinesin-13 family conserved KVD residues, and as predicted from the crossbow model, mutating these residues changes the orientation and mobility of KLP10AHDs interacting with the microtubule.

Structural Model for Tubulin Recognition and Deformation by Kinesin-13 Microtubule Depolymerases.,Asenjo AB, Chatterjee C, Tan D, Depaoli V, Rice WJ, Diaz-Avalos R, Silvestry M, Sosa H Cell Rep. 2013 Feb 19. pii: S2211-1247(13)00054-5. doi:, 10.1016/j.celrep.2013.01.030. PMID:23434508^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rogers GC, Rogers SL, Schwimmer TA, Ems-McClung SC, Walczak CE, Vale RD, Scholey JM, Sharp DJ. Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature. 2004 Jan 22;427(6972):364-70. Epub 2003 Dec 14. PMID:14681690 doi:10.1038/nature02256
↑ Asenjo AB, Chatterjee C, Tan D, Depaoli V, Rice WJ, Diaz-Avalos R, Silvestry M, Sosa H. Structural Model for Tubulin Recognition and Deformation by Kinesin-13 Microtubule Depolymerases. Cell Rep. 2013 Feb 19. pii: S2211-1247(13)00054-5. doi:, 10.1016/j.celrep.2013.01.030. PMID:23434508 doi:http://dx.doi.org/10.1016/j.celrep.2013.01.030

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j2u"

@@ Line 3: / Line 3: @@
 <SX load='3j2u' size='340' side='right' viewer='molstar' caption='[[3j2u]], [[Resolution|resolution]] 10.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3j2u]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Drome Drome]. The July 2014 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Microtubules''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2014_7 10.2210/rcsb_pdb/mom_2014_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J2U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J2U FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3j2u]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Drome Drome]. The July 2014 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Microtubules''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2014_7 10.2210/rcsb_pdb/mom_2014_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J2U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J2U FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Klp10A, CG1453 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j2u OCA], [http://pdbe.org/3j2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j2u RCSB], [http://www.ebi.ac.uk/pdbsum/3j2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j2u ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j2u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j2u OCA], [http://pdbe.org/3j2u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j2u RCSB], [http://www.ebi.ac.uk/pdbsum/3j2u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j2u ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 ==See Also==
 *[[Kinesin 3D Structures|Kinesin 3D Structures]]
-*[[Tubulin|Tubulin]]
+*[[Tubulin 3D Structures|Tubulin 3D Structures]]
 == References ==
 <references/>

3j2u

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Revision as of 17:49, 10 April 2020

Kinesin-13 KLP10A HD in complex with CS-tubulin and a microtubule

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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