3j4j

From Proteopedia

(Difference between revisions)

Revision as of 17:55, 10 April 2020

Model of full-length T. thermophilus Translation Initiation Factor 2 refined against its cryo-EM density from a 30S Initiation Complex map

3j4j, resolution 11.50Å

Structural highlights

3j4j is a 1 chain structure with sequence from Thet8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4b3x, 4b48, 1d1n, 4kjz
Gene:	infB, TTHA0699 (THET8)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IF2_THET8] One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex (By similarity).

Publication Abstract from PubMed

Translation initiation factor 2 (IF2) promotes 30S initiation complex (IC) formation and 50S subunit joining, which produces the 70S IC. The architecture of full-length IF2, determined by small angle X-ray diffraction and cryo electron microscopy, reveals a more extended conformation of IF2 in solution and on the ribosome than in the crystal. The N-terminal domain is only partially visible in the 30S IC, but in the 70S IC, it stabilizes interactions between IF2 and the L7/L12 stalk of the 50S, and on its deletion, proper N-formyl-methionyl(fMet)-tRNA(fMet) positioning and efficient transpeptidation are affected. Accordingly, fast kinetics and single-molecule fluorescence data indicate that the N terminus promotes 70S IC formation by stabilizing the productive sampling of the 50S subunit during 30S IC joining. Together, our data highlight the dynamics of IF2-dependent ribosomal subunit joining and the role played by the N terminus of IF2 in this process.

Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor.,Simonetti A, Marzi S, Billas IM, Tsai A, Fabbretti A, Myasnikov AG, Roblin P, Vaiana AC, Hazemann I, Eiler D, Steitz TA, Puglisi JD, Gualerzi CO, Klaholz BP Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15656-61. doi:, 10.1073/pnas.1309578110. Epub 2013 Sep 12. PMID:24029017^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Simonetti A, Marzi S, Billas IM, Tsai A, Fabbretti A, Myasnikov AG, Roblin P, Vaiana AC, Hazemann I, Eiler D, Steitz TA, Puglisi JD, Gualerzi CO, Klaholz BP. Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor. Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15656-61. doi:, 10.1073/pnas.1309578110. Epub 2013 Sep 12. PMID:24029017 doi:10.1073/pnas.1309578110

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j4j"

@@ Line 3: / Line 3: @@
 <SX load='3j4j' size='340' side='right' viewer='molstar' caption='[[3j4j]], [[Resolution|resolution]] 11.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3j4j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J4J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3j4j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J4J FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4b3x|4b3x]], [[4b48|4b48]], [[1d1n|1d1n]], [[4kjz|4kjz]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">infB, TTHA0699 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4j OCA], [http://pdbe.org/3j4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j4j RCSB], [http://www.ebi.ac.uk/pdbsum/3j4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j4j ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4j OCA], [http://pdbe.org/3j4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j4j RCSB], [http://www.ebi.ac.uk/pdbsum/3j4j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j4j ProSAT]</span></td></tr>
 </table>
 == Function ==

3j4j

From Proteopedia

Revision as of 17:55, 10 April 2020

Model of full-length T. thermophilus Translation Initiation Factor 2 refined against its cryo-EM density from a 30S Initiation Complex map

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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