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3lu0

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Revision as of 18:40, 10 April 2020

Molecular model of Escherichia coli core RNA polymerase

3lu0, resolution 11.20Å

Structural highlights

3lu0 is a 5 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3lti
Gene:	b3295, JW3257, pez, phs, rpoA, sez (ECOLI), b3987, groN, JW3950, nitB, rif, ron, rpoB, rpoC, stl, stv, tabD (ECOLI), b3988, JW3951, rpoC, rpoD, tabB (ECOLI), b3649, JW3624, rpoZ (ECOLI)
Activity:	DNA-directed RNA polymerase, with EC number 2.7.7.6
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPOZ_ECOLI] Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.[HAMAP-Rule:MF_00366] [RPOA_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059] [RPOC_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01322] [RPOB_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_01321]

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The Escherichia coli transcription system is the best characterized from a biochemical and genetic point of view and has served as a model system. Nevertheless, a molecular understanding of the details of E. coli transcription and its regulation, and therefore its full exploitation as a model system, has been hampered by the absence of high-resolution structural information on E. coli RNA polymerase (RNAP). We use a combination of approaches, including high-resolution X-ray crystallography, ab initio structural prediction, homology modeling, and single-particle cryo-electron microscopy, to generate complete atomic models of E. coli core RNAP and an E. coli RNAP ternary elongation complex. The detailed and comprehensive structural descriptions can be used to help interpret previous biochemical and genetic data in a new light and provide a structural framework for designing experiments to understand the function of the E. coli lineage-specific insertions and their role in the E. coli transcription program.

Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.,Opalka N, Brown J, Lane WJ, Twist KA, Landick R, Asturias FJ, Darst SA PLoS Biol. 2010 Sep 14;8(9). pii: e1000483. PMID:20856905^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Opalka N, Brown J, Lane WJ, Twist KA, Landick R, Asturias FJ, Darst SA. Complete structural model of Escherichia coli RNA polymerase from a hybrid approach. PLoS Biol. 2010 Sep 14;8(9). pii: e1000483. PMID:20856905 doi:10.1371/journal.pbio.1000483

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3lu0"

@@ Line 3: / Line 3: @@
 <SX load='3lu0' size='340' side='right' viewer='molstar' caption='[[3lu0]], [[Resolution|resolution]] 11.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3lu0]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LU0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LU0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3lu0]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LU0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3LU0 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lti|3lti]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b3295, JW3257, pez, phs, rpoA, sez ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), b3987, groN, JW3950, nitB, rif, ron, rpoB, rpoC, stl, stv, tabD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), b3988, JW3951, rpoC, rpoD, tabB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), b3649, JW3624, rpoZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lu0 OCA], [http://pdbe.org/3lu0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lu0 RCSB], [http://www.ebi.ac.uk/pdbsum/3lu0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lu0 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3lu0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lu0 OCA], [http://pdbe.org/3lu0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3lu0 RCSB], [http://www.ebi.ac.uk/pdbsum/3lu0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3lu0 ProSAT]</span></td></tr>
 </table>
 == Function ==

3lu0

From Proteopedia

Revision as of 18:40, 10 April 2020

Molecular model of Escherichia coli core RNA polymerase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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