5n8y

From Proteopedia

(Difference between revisions)

Revision as of 21:11, 10 April 2020

KaiCBA circadian clock backbone model based on a Cryo-EM density

5n8y, resolution 4.70Å

Structural highlights

5n8y is a 24 chain structure with sequence from Pcc 6301. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	kaiC, Synpcc7942_1216, see0011 (PCC 6301), kaiB, Synpcc7942_1217, see0010 (PCC 6301), kaiA, Synpcc7942_1218, see0009 (PCC 6301)
Activity:	Non-specific serine/threonine protein kinase, with EC number 2.7.11.1
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KAIC_SYNE7] Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction.^[1] ^[2] [KAIA_SYNE7] Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation.^[3] ^[4] ^[5] ^[6] [KAIB_SYNE7] Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-non-specific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it decreases the phosphorylation status of KaiC. It has no effect on KaiC by itself, but instead need the presence of both KaiA and KaiC, suggesting that it acts by antagonizing the interaction between KaiA and KaiC.^[7] ^[8] ^[9] ^[10]

Publication Abstract from PubMed

Cyanobacteria have a robust circadian oscillator, known as the Kai system. Reconstituted from the purified protein components KaiC, KaiB, and KaiA, it can tick autonomously in the presence of adenosine 5'-triphosphate (ATP). The KaiC hexamers enter a natural 24-hour reaction cycle of autophosphorylation and assembly with KaiB and KaiA in numerous diverse forms. We describe the preparation of stoichiometrically well-defined assemblies of KaiCB and KaiCBA, as monitored by native mass spectrometry, allowing for a structural characterization by single-particle cryo-electron microscopy and mass spectrometry. Our data reveal details of the interactions between the Kai proteins and provide a structural basis to understand periodic assembly of the protein oscillator.

Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state.,Snijder J, Schuller JM, Wiegard A, Lossl P, Schmelling N, Axmann IM, Plitzko JM, Forster F, Heck AJ Science. 2017 Mar 17;355(6330):1181-1184. doi: 10.1126/science.aag3218. Epub 2017, Mar 16. PMID:28302852^[11]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ishiura M, Kutsuna S, Aoki S, Iwasaki H, Andersson CR, Tanabe A, Golden SS, Johnson CH, Kondo T. Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria. Science. 1998 Sep 4;281(5382):1519-23. PMID:9727980
↑ Nakahira Y, Katayama M, Miyashita H, Kutsuna S, Iwasaki H, Oyama T, Kondo T. Global gene repression by KaiC as a master process of prokaryotic circadian system. Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):881-5. Epub 2004 Jan 6. PMID:14709675 doi:10.1073/pnas.0307411100
↑ Iwasaki H, Nishiwaki T, Kitayama Y, Nakajima M, Kondo T. KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria. Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15788-93. Epub 2002 Oct 21. PMID:12391300 doi:http://dx.doi.org/10.1073/pnas.222467299
↑ Xu Y, Mori T, Johnson CH. Cyanobacterial circadian clockwork: roles of KaiA, KaiB and the kaiBC promoter in regulating KaiC. EMBO J. 2003 May 1;22(9):2117-26. PMID:12727878 doi:10.1093/emboj/cdg168
↑ Kitayama Y, Iwasaki H, Nishiwaki T, Kondo T. KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial circadian clock system. EMBO J. 2003 May 1;22(9):2127-34. PMID:12727879 doi:10.1093/emboj/cdg212
↑ Ishiura M, Kutsuna S, Aoki S, Iwasaki H, Andersson CR, Tanabe A, Golden SS, Johnson CH, Kondo T. Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria. Science. 1998 Sep 4;281(5382):1519-23. PMID:9727980
↑ Ishiura M, Kutsuna S, Aoki S, Iwasaki H, Andersson CR, Tanabe A, Golden SS, Johnson CH, Kondo T. Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria. Science. 1998 Sep 4;281(5382):1519-23. PMID:9727980
↑ Xu Y, Mori T, Johnson CH. Cyanobacterial circadian clockwork: roles of KaiA, KaiB and the kaiBC promoter in regulating KaiC. EMBO J. 2003 May 1;22(9):2117-26. PMID:12727878 doi:10.1093/emboj/cdg168
↑ Kitayama Y, Iwasaki H, Nishiwaki T, Kondo T. KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial circadian clock system. EMBO J. 2003 May 1;22(9):2127-34. PMID:12727879 doi:10.1093/emboj/cdg212
↑ Nakahira Y, Katayama M, Miyashita H, Kutsuna S, Iwasaki H, Oyama T, Kondo T. Global gene repression by KaiC as a master process of prokaryotic circadian system. Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):881-5. Epub 2004 Jan 6. PMID:14709675 doi:10.1073/pnas.0307411100
↑ Snijder J, Schuller JM, Wiegard A, Lossl P, Schmelling N, Axmann IM, Plitzko JM, Forster F, Heck AJ. Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state. Science. 2017 Mar 17;355(6330):1181-1184. doi: 10.1126/science.aag3218. Epub 2017, Mar 16. PMID:28302852 doi:http://dx.doi.org/10.1126/science.aag3218

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5n8y"

@@ Line 3: / Line 3: @@
 <SX load='5n8y' size='340' side='right' viewer='molstar' caption='[[5n8y]], [[Resolution|resolution]] 4.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5n8y]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pcc_6301 Pcc 6301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N8Y FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5n8y]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Pcc_6301 Pcc 6301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N8Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N8Y FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">kaiC, Synpcc7942_1216, see0011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32046 PCC 6301]), kaiB, Synpcc7942_1217, see0010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32046 PCC 6301]), kaiA, Synpcc7942_1218, see0009 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32046 PCC 6301])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n8y OCA], [http://pdbe.org/5n8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n8y RCSB], [http://www.ebi.ac.uk/pdbsum/5n8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n8y ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n8y OCA], [http://pdbe.org/5n8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n8y RCSB], [http://www.ebi.ac.uk/pdbsum/5n8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n8y ProSAT]</span></td></tr>
 </table>
 == Function ==

5n8y

From Proteopedia

Revision as of 21:11, 10 April 2020

KaiCBA circadian clock backbone model based on a Cryo-EM density

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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