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5oh0

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Revision as of 21:28, 10 April 2020

The Cryo-Electron Microscopy Structure of the Type 1 Chaperone-Usher Pilus Rod

5oh0, resolution 4.20Å

Structural highlights

5oh0 is a 6 chain structure with sequence from Escherichia coli j96. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	fimA, pilA, b4314, JW4277 (Escherichia coli J96)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FIMA1_ECOLI] Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.

Publication Abstract from PubMed

Adhesive chaperone-usher pili are long, supramolecular protein fibers displayed on the surface of many bacterial pathogens. The type 1 and P pili of uropathogenic Escherichia coli (UPEC) play important roles during urinary tract colonization, mediating attachment to the bladder and kidney, respectively. The biomechanical properties of the helical pilus rods allow them to reversibly uncoil in response to flow-induced forces, allowing UPEC to retain a foothold in the unique and hostile environment of the urinary tract. Here we provide the 4.2-A resolution cryo-EM structure of the type 1 pilus rod, which together with the previous P pilus rod structure rationalizes the remarkable "spring-like" properties of chaperone-usher pili. The cryo-EM structure of the type 1 pilus rod differs in its helical parameters from the structure determined previously by a hybrid approach. We provide evidence that these structural differences originate from different quaternary structures of pili assembled in vivo and in vitro.

The Cryoelectron Microscopy Structure of the Type 1 Chaperone-Usher Pilus Rod.,Hospenthal MK, Zyla D, Costa TRD, Redzej A, Giese C, Lillington J, Glockshuber R, Waksman G Structure. 2017 Nov 7. pii: S0969-2126(17)30332-5. doi:, 10.1016/j.str.2017.10.004. PMID:29129382^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hospenthal MK, Zyla D, Costa TRD, Redzej A, Giese C, Lillington J, Glockshuber R, Waksman G. The Cryoelectron Microscopy Structure of the Type 1 Chaperone-Usher Pilus Rod. Structure. 2017 Nov 7. pii: S0969-2126(17)30332-5. doi:, 10.1016/j.str.2017.10.004. PMID:29129382 doi:http://dx.doi.org/10.1016/j.str.2017.10.004

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5oh0"

@@ Line 3: / Line 3: @@
 <SX load='5oh0' size='340' side='right' viewer='molstar' caption='[[5oh0]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5oh0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_j96 Escherichia coli j96]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OH0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OH0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5oh0]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_j96 Escherichia coli j96]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OH0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OH0 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fimA, pilA, b4314, JW4277 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1206108 Escherichia coli J96])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oh0 OCA], [http://pdbe.org/5oh0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oh0 RCSB], [http://www.ebi.ac.uk/pdbsum/5oh0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oh0 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5oh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oh0 OCA], [http://pdbe.org/5oh0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oh0 RCSB], [http://www.ebi.ac.uk/pdbsum/5oh0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oh0 ProSAT]</span></td></tr>
 </table>
 == Function ==

5oh0

From Proteopedia

Revision as of 21:28, 10 April 2020

The Cryo-Electron Microscopy Structure of the Type 1 Chaperone-Usher Pilus Rod

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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