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5tjt

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Revision as of 21:45, 10 April 2020

T5 bacteriophage major capsid protein - one PB8 hexon

5tjt, resolution 9.00Å

Structural highlights

5tjt is a 6 chain structure with sequence from Escherichia phage t5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CAPSD_BPT5] Major capsid protein that self-associates to form 120 hexamers and 11 pentamers, building the T=13 icosahedral capsid which about 860 Angstroms in diameter. Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function. The capsid gains its final stability through the reorganization of the subunits that takes place upon expansion. DNA encapsidation through the portal triggers capsid expansion and the binding of the decoration protein to the capsid exterior.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Bacteriophage capsids constitute icosahedral shells of exceptional stability that protect the viral genome. Many capsids display on their surface decoration proteins whose structure and function remain largely unknown. The decoration protein pb10 of phage T5 binds at the centre of the 120 hexamers formed by the major capsid protein. Here we determined the 3D structure of pb10 and investigated its capsid-binding properties using NMR, SAXS, cryoEM and SPR. Pb10 consists of an alpha-helical capsid-binding domain and an Ig-like domain exposed to the solvent. It binds to the T5 capsid with a remarkably high affinity and its binding kinetics is characterized by a very slow dissociation rate. We propose that the conformational exchange events observed in the capsid-binding domain enable rearrangements upon binding that contribute to the quasi-irreversibility of the pb10-capsid interaction. Moreover we show that pb10 binding is a highly cooperative process, which favours immediate rebinding of newly dissociated pb10 to the 120 hexamers of the capsid protein. In extreme conditions, pb10 protects the phage from releasing its genome. We conclude that pb10 may function to reinforce the capsid thus favouring phage survival in harsh environments.

High affinity anchoring of the decoration protein pb10 onto the bacteriophage T5 capsid.,Vernhes E, Renouard M, Gilquin B, Cuniasse P, Durand D, England P, Hoos S, Huet A, Conway JF, Glukhov A, Ksenzenko V, Jacquet E, Nhiri N, Zinn-Justin S, Boulanger P Sci Rep. 2017 Feb 6;7:41662. doi: 10.1038/srep41662. PMID:28165000^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Huet A, Conway JF, Letellier L, Boulanger P. In vitro assembly of the T=13 procapsid of bacteriophage T5 with its scaffolding domain. J Virol. 2010 Sep;84(18):9350-8. doi: 10.1128/JVI.00942-10. Epub 2010 Jun 23. PMID:20573812 doi:http://dx.doi.org/10.1128/JVI.00942-10
↑ Preux O, Durand D, Huet A, Conway JF, Bertin A, Boulogne C, Drouin-Wahbi J, Trevarin D, Perez J, Vachette P, Boulanger P. A two-state cooperative expansion converts the procapsid shell of bacteriophage T5 into a highly stable capsid isomorphous to the final virion head. J Mol Biol. 2013 Jun 12;425(11):1999-2014. doi: 10.1016/j.jmb.2013.03.002. Epub, 2013 Mar 13. PMID:23500494 doi:http://dx.doi.org/10.1016/j.jmb.2013.03.002
↑ Huet A, Duda RL, Hendrix RW, Boulanger P, Conway JF. Correct Assembly of the Bacteriophage T5 Procapsid Requires Both the Maturation Protease and the Portal Complex. J Mol Biol. 2016 Jan 16;428(1):165-81. doi: 10.1016/j.jmb.2015.11.019. Epub 2015 , Nov 23. PMID:26616586 doi:http://dx.doi.org/10.1016/j.jmb.2015.11.019
↑ Vernhes E, Renouard M, Gilquin B, Cuniasse P, Durand D, England P, Hoos S, Huet A, Conway JF, Glukhov A, Ksenzenko V, Jacquet E, Nhiri N, Zinn-Justin S, Boulanger P. High affinity anchoring of the decoration protein pb10 onto the bacteriophage T5 capsid. Sci Rep. 2017 Feb 6;7:41662. doi: 10.1038/srep41662. PMID:28165000 doi:http://dx.doi.org/10.1038/srep41662

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5tjt"

@@ Line 3: / Line 3: @@
 <SX load='5tjt' size='340' side='right' viewer='molstar' caption='[[5tjt]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5tjt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_phage_t5 Escherichia phage t5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TJT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TJT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5tjt]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_phage_t5 Escherichia phage t5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TJT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5TJT FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tjt OCA], [http://pdbe.org/5tjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tjt RCSB], [http://www.ebi.ac.uk/pdbsum/5tjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tjt ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5tjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tjt OCA], [http://pdbe.org/5tjt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tjt RCSB], [http://www.ebi.ac.uk/pdbsum/5tjt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tjt ProSAT]</span></td></tr>
 </table>
 == Function ==

5tjt

From Proteopedia

Revision as of 21:45, 10 April 2020

T5 bacteriophage major capsid protein - one PB8 hexon

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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