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5u0a

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Revision as of 21:49, 10 April 2020

CRISPR RNA-guided surveillance complex

5u0a, resolution 3.30Å

Structural highlights

5u0a is a 14 chain structure with sequence from Thefy. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	5u07
Gene:	Tfu_1588 (THEFY), Tfu_1592 (THEFY), Tfu_1590 (THEFY), Tfu_1591 (THEFY), Tfu_1589 (THEFY)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Type I CRISPR systems feature a sequential dsDNA target searching and degradation process, by crRNA-displaying Cascade and nuclease-helicase fusion enzyme Cas3, respectively. Here we present two cryo-EM snapshots of the Thermobifida fusca type I-E Cascade: (1) unwinding 11 bp of dsDNA at the seed-sequence region to scout for sequence complementarity, and (2) further unwinding of the entire protospacer to form a full R-loop. These structures provide the much-needed temporal and spatial resolution to resolve key mechanistic steps leading to Cas3 recruitment. In the early steps, PAM recognition causes severe DNA bending, leading to spontaneous DNA unwinding to form a seed-bubble. The full R-loop formation triggers conformational changes in Cascade, licensing Cas3 to bind. The same process also generates a bulge in the non-target DNA strand, enabling its handover to Cas3 for cleavage. The combination of both negative and positive checkpoints ensures stringent yet efficient target degradation in type I CRISPR-Cas systems.

Structure Basis for Directional R-loop Formation and Substrate Handover Mechanisms in Type I CRISPR-Cas System.,Xiao Y, Luo M, Hayes RP, Kim J, Ng S, Ding F, Liao M, Ke A Cell. 2017 Jun 29;170(1):48-60.e11. doi: 10.1016/j.cell.2017.06.012. PMID:28666122^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xiao Y, Luo M, Hayes RP, Kim J, Ng S, Ding F, Liao M, Ke A. Structure Basis for Directional R-loop Formation and Substrate Handover Mechanisms in Type I CRISPR-Cas System. Cell. 2017 Jun 29;170(1):48-60.e11. doi: 10.1016/j.cell.2017.06.012. PMID:28666122 doi:http://dx.doi.org/10.1016/j.cell.2017.06.012

1 Structural highlights
2 Publication Abstract from PubMed
3 See Also
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5u0a"

@@ Line 3: / Line 3: @@
 <SX load='5u0a' size='340' side='right' viewer='molstar' caption='[[5u0a]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5u0a]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Thefy Thefy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U0A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5u0a]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Thefy Thefy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U0A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5U0A FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5u07|5u07]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tfu_1588 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY]), Tfu_1592 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY]), Tfu_1590 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY]), Tfu_1591 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY]), Tfu_1589 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u0a OCA], [http://pdbe.org/5u0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u0a RCSB], [http://www.ebi.ac.uk/pdbsum/5u0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u0a ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u0a OCA], [http://pdbe.org/5u0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u0a RCSB], [http://www.ebi.ac.uk/pdbsum/5u0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u0a ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">

5u0a

From Proteopedia

Revision as of 21:49, 10 April 2020

CRISPR RNA-guided surveillance complex

Structural highlights

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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