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5u6y

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Revision as of 21:51, 10 April 2020

Pseudo-atomic model of the CaMKIIa holoenzyme.

5u6y, resolution 20.00Å

Structural highlights

5u6y is a 12 chain structure with sequence from Buffalo rat. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	Camk2a (Buffalo rat)
Activity:	Calcium/calmodulin-dependent protein kinase, with EC number 2.7.11.17
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KCC2A_RAT] CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.^[1]

Publication Abstract from PubMed

The Ca2+/calmodulin-dependent protein kinase II (CaMKII) assembles into large 12-meric holoenzymes, which is thought to enable regulatory processes required for synaptic plasticity underlying learning, memory and cognition. Here we used single particle electron microscopy (EM) to determine a pseudoatomic model of the CaMKIIalpha holoenzyme in an extended and activation-competent conformation. The holoenzyme is organized by a rigid central hub complex, while positioning of the kinase domains is highly flexible, revealing dynamic holoenzymes ranging from 15-35 nm in diameter. While most kinase domains are ordered independently, approximately 20% appear to form dimers and <3% are consistent with a compact conformation. An additional level of plasticity is revealed by a small fraction of bona-fide 14-mers (<4%) that may enable subunit exchange. Biochemical and cellular FRET studies confirm that the extended state of CaMKIIalpha resolved by EM is the predominant form of the holoenzyme, even under molecular crowding conditions.

The CaMKII holoenzyme structure in activation-competent conformations.,Myers JB, Zaegel V, Coultrap SJ, Miller AP, Bayer KU, Reichow SL Nat Commun. 2017 Jun 7;8:15742. doi: 10.1038/ncomms15742. PMID:28589927^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Krapivinsky G, Medina I, Krapivinsky L, Gapon S, Clapham DE. SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation. Neuron. 2004 Aug 19;43(4):563-74. PMID:15312654 doi:10.1016/j.neuron.2004.08.003
↑ Myers JB, Zaegel V, Coultrap SJ, Miller AP, Bayer KU, Reichow SL. The CaMKII holoenzyme structure in activation-competent conformations. Nat Commun. 2017 Jun 7;8:15742. doi: 10.1038/ncomms15742. PMID:28589927 doi:http://dx.doi.org/10.1038/ncomms15742

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5u6y"

@@ Line 3: / Line 3: @@
 <SX load='5u6y' size='340' side='right' viewer='molstar' caption='[[5u6y]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5u6y]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U6Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U6Y FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5u6y]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U6Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5U6Y FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Camk2a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calcium/calmodulin-dependent_protein_kinase Calcium/calmodulin-dependent protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.17 2.7.11.17] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u6y OCA], [http://pdbe.org/5u6y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u6y RCSB], [http://www.ebi.ac.uk/pdbsum/5u6y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u6y ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5u6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u6y OCA], [http://pdbe.org/5u6y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u6y RCSB], [http://www.ebi.ac.uk/pdbsum/5u6y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u6y ProSAT]</span></td></tr>
 </table>
 == Function ==

5u6y

From Proteopedia

Revision as of 21:51, 10 April 2020

Pseudo-atomic model of the CaMKIIa holoenzyme.

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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